Poly(dimethylsiloxane) microchip electrophoresis with contactless conductivity detection for measurement of chemical warfare agent degradation products

被引：27

作者：

Ding, Yongsheng ^{[1
]}

Garcia, Carlos D. ^{[2
]}

Rogers, Kim R. ^{[1
]}

机构：

[1] US EPA, Natl Exposure Res Lab LV, Las Vegas, NV 89119 USA

[2] Univ Texas San Antonio, Dept Chem, San Antonio, TX USA

来源：

ANALYTICAL LETTERS | 2008年 / 41卷 / 02期

关键词：

chemical warfare agent degradation products; alkyl methylphosphonic acids; microchip capillary electrophoresis; contactless conductivity detection; poly(dimethylsiloxane);

D O I：

10.1080/00032710701792943

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The applicability of a poly(dimethylsiloxane) (PDMS) microfluidic device with contactless conductivity detection for the determination of organophosphonate nerve agent degradation products is reported. Five alkyl methylphosphonic acids, isopropyl methylphosphonic acid (IMPA), pinacolyl methylphosphonic acid (PMPA), O-ethyl-N,N-dimethyl phosphoramidate (EDPA), ethyl methylphosphonic acid (EMPA), and methylphosphonic acid (MPA), (degradation products of Sarin, Soman, Tuban and VX nerve agents) were analyzed by microchip capillary electrophoresis. Experimental conditions for the separation and detection processes have been optimized to yield well-defined separation and high sensitivity. Under optimal conditions, analyses were completed in less than 2min. Linear relations between concentration and peak heights were obtained with detection limits in the 1.3-4.5mg/l range and precision values for the peak heights were in the range of 3.4-6.1% RSD. Applicability of this method for natural (lake and tap) water samples was also demonstrated. Compared to conventional analytical methods, this miniaturized system offers promise for on-site monitoring of degradation products of chemical warfare agents, with advantages of cost effective construction, simple operation, portability, and minimum sample consumption.

引用

页码：335 / 350

页数：16

共 47 条

[1]

Becker H, 2000, ELECTROPHORESIS, V21, P12, DOI 10.1002/(SICI)1522-2683(20000101)21:1<12::AID-ELPS12>3.0.CO

[2]

2-7

[3] Fabrication of porous polymer monoliths in polymeric microfluidic chips as an electrospray emitter for direct coupling to mass spectrometry [J].

Bedair, MF ;

Oleschuk, RD .

ANALYTICAL CHEMISTRY, 2006, 78 (04) :1130-1138

[4] Sample filtration, concentration, and separation integrated on microfluidic devices [J].

Broyles, BS ;

Jacobson, SC ;

Ramsey, JM .

ANALYTICAL CHEMISTRY, 2003, 75 (11) :2761-2767

[5] Single-step fabrication and characterization of photonic crystal biosensors with polymer microfluidic channels [J].

Choi, Charles J. ;

Cunningham, Brian T. .

LAB ON A CHIP, 2006, 6 (10) :1373-1380

[6] Liquid chromatography electrospray tandem mass spectrometric and desorption electrospray ionization tandem mass spectrometric analysis of chemical warfare agents in office media typically collected during a forensic investigation [J].

D'Agostino, PA ;

Hancock, JR ;

Chenier, CL ;

Lepage, CRJ .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1110 (1-2) :86-94

[7]

da Silva JAF, 1998, ANAL CHEM, V70, P4339

[8] Pulsed amperometric detection with poly(dimethylsiloxane)-fabricated capillary electrophoresis microchips for the determination of EPA priority pollutants [J].

Ding, YS ;

Garcia, CD .

ANALYST, 2006, 131 (02) :208-214

[9] Separation of proteins on surface-modified poly(dimethylsiloxane) microfluidic devices [J].

Dou, YH ;

Bao, N ;

Xu, JJ ;

Meng, F ;

Chen, HY .

ELECTROPHORESIS, 2004, 25 (17) :3024-3031

[10] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) [J].

Duffy, DC ;

McDonald, JC ;

Schueller, OJA ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 1998, 70 (23) :4974-4984

← 1 2 3 4 5 →