Molecular typing of Mycobacterium tuberculosis by mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis, a more accurate method for identifying epidemiological links between patients with tuberculosis

被引：74

作者：

van Deutekom, H

Supply, P

de Haas, PEW

Willery, E

Hoijng, SP

Locht, C

Coutinho, RA

van Soolingen, D

机构：

[1] Municipal Hlth Serv, Dept TB Control, Amsterdam, Netherlands

[2] Inst Pasteur, INSERM, Lab Mecan Mol Pathogenese Bacterienne, U629, F-59019 Lille, France

[3] Natl Inst Publ Hlth & Environm, Diagnost Lab Infect Dis & Perinatal Screening, NL-3720 BA Bilthoven, Netherlands

[4] Univ Amsterdam, Acad Med Ctr, Dept Human Retrovirol, NL-1105 AZ Amsterdam, Netherlands

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 09期

关键词：

D O I：

10.1128/JCM.43.9.4473-4479.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

IS6110 fingerprinting of Mycobacterium tuberculosis is the standard identification method in studies on transmission of tuberculosis. However, intensive epidemiological investigation may fail to confirm transmission links between patients clustered by IS6110-restriction fragment length polymorphism (RFLP) typing. We applied typing based on variable numbers of tandem repeats (VNTRs) of mycobacterial interspersed repetitive units (MIRUs) to isolates from 125 patients in 42 IS6110 clusters, for which thorough epidemiological data were available, to investigate the potential of this method in distinguishing epidemiologically linked from nonlinked patients. Of seven IS6110 clusters without epidemiological links, five were split by MIRU-VNTR typing, while nearly all IS6110 clusters with proven or likely links displayed conserved MIRU-VNTR types. These results provide molecular evidence that not all clusters determined on the basis of multibanded IS6110 RFLP patterns necessarily reflect transmission of tuberculosis. They support the use of MIRU-VNTR typing as a more reliable and faster method for transmission analysis.

引用

收藏

页码：4473 / 4479

页数：7

相关论文

共 39 条

[1] Transmission of a highly drug-resistant strain (strain W1) of Mycobacterium tuberculosis - Community outbreak and nosocomial transmission via a contaminated bronchoscope [J].

Agerton, T ;

Valway, S ;

Gore, B ;

Pozsik, C ;

Plikaytis, B ;

Woodley, C ;

Onorato, I .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1997, 278 (13) :1073-1077

[2] TRANSMISSION OF TUBERCULOSIS IN NEW-YORK-CITY - AN ANALYSIS BY DNA-FINGERPRINTING AND CONVENTIONAL EPIDEMIOLOGIC METHODS [J].

ALLAND, D ;

KALKUT, GE ;

MOSS, AR ;

MCADAM, RA ;

HAHN, JA ;

BOSWORTH, W ;

DRUCKER, E ;

BLOOM, BR .

NEW ENGLAND JOURNAL OF MEDICINE, 1994, 330 (24) :1710-1716

[3] Utility of fast mycobacterial interspersed repetitive unit-variable number tandem repeat genotyping in clinical mycobacteriological analysis [J].

Allix, C ;

Supply, P ;

Fauville-Dufaux, M .

CLINICAL INFECTIOUS DISEASES, 2004, 39 (06) :783-789

[4] Comparison of variable number tandem repeat and IS6110-restriction fragment length polymorphism analyses for discrimination of high- and low-copy-number IS6110 Mycobacterium tuberculosis isolates [J].

Barlow, REL ;

Gascoyne-Binzi, DM ;

Gillespie, SH ;

Dickens, A ;

Qamer, S ;

Hawkey, PM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (07) :2453-2457

[5] The challenges of tuberculosis on antimicrobial resistance [J].

REVISTA DE SAUDE PUBLICA, 2011, 45 (05) :997-1000

[6] Outbreak of tuberculosis associated with a floating card game in the rural south: Lessons for tuberculosis contact investigations [J].

Bock, NN ;

Mallory, JP ;

Mobley, N ;

DeVoe, B ;

Taylor, BB .

CLINICAL INFECTIOUS DISEASES, 1998, 27 (05) :1221-1226

[7] Interpretation of restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolates from a state with a large rural population [J].

Braden, CR ;

Templeton, GL ;

Cave, MD ;

Valway, S ;

Onorato, IM ;

Castro, KG ;

Moers, D ;

Yang, ZH ;

Stead, WW ;

Bates, JH .

JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (06) :1446-1452

[8] Epidemiological evidence of the spread of a Mycobacterium tuberculosis strain of the Beijing genotype on Gran Canaria Island [J].

Caminero, JA ;

Pena, MJ ;

Campos-Herrero, MI ;

Rodríguez, JC ;

Garcia, I ;

Cabrera, P ;

Lafoz, C ;

Samper, S ;

Takiff, H ;

Afonso, O ;

Pavón, JM ;

Torres, MJ ;

van Soolingen, D ;

Enarson, DA ;

Martin, C .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 164 (07) :1165-1170

[9] Variable-number-tandem repeat typing of mycobacterium tuberculosis isolates with low copy numbers of IS6110 by using mycobacterial interspersed repetitive units [J].

Cowan, LS ;

Mosher, L ;

Diem, L ;

Massey, JP ;

Crawford, JT .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (05) :1592-1602

[10] AN OUTBREAK OF TUBERCULOSIS WITH ACCELERATED PROGRESSION AMONG PERSONS INFECTED WITH THE HUMAN-IMMUNODEFICIENCY-VIRUS - AN ANALYSIS USING RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS [J].

DALEY, CL ;

SMALL, PM ;

SCHECTER, GF ;

SCHOOLNIK, GK ;

MCADAM, RA ;

JACOBS, WR ;

HOPEWELL, PC .

NEW ENGLAND JOURNAL OF MEDICINE, 1992, 326 (04) :231-235

← 1 2 3 4 →