A hypervariable N-terminal region of Yersinia LcrV determines Toll-like receptor 2-mediated IL-10 induction and mouse virulence

被引:84
作者
Sing, A
Reithmeier-Rost, D
Granfors, K
Hill, J
Roggenkamp, A
Heesemann, J
机构
[1] Max Von Pettenkofer Inst Hyg & Med Microbiol, Lehrstuhl Bakteriol, D-80336 Munich, Germany
[2] Natl Publ Hlth Inst, Dept Bacterial & Inflammatory Dis, FIN-20520 Turku, Finland
[3] Def Sci & Technol Lab, Salisbury SP4 0JQ, Wilts, England
关键词
pathogenesis;
D O I
10.1073/pnas.0504728102
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The virulence antigen LcrV of Yersinia enterocolitica 0:8 induces IL-10 in macrophages via Toll-like receptor 2 (TLR2). The TLR2-active region of LcrV is localized within its N-terminal amino acids (aa) 31-57. Sequencing of codons 25-92 of the lcrV gene from 59 strains of the three pathogenic Yersinia species revealed a hypervariable hotspot within as 40-61. According to these sequence differences, seven LcrV groups were identified, with Y. pestis and Y. pseudotuberculosis represented in group I and the other six distributed within Y. enterocolitica. By testing LcrV sequence-derived synthetic oligopeptides of all seven LcrV groups in CD14/TLR2-transfected human embryonic kidney 293 cells, we found the highest TLR2 activity with a peptide derived from group IV comprising exclusively Y. enterocolitica 0:8 strains. These findings were verified in murine peritoneal macrophages by using recombinant LcrV truncates representing as 1-130 from different Yersinia spp. By systematically replacing charged as residues by glutamine in synthetic oligopeptides, we show that the K42Q substitution leads to abrogation of TLR2 activity in both in vitro cell systems. This K42Q substitution was introduced in the lcrV gene from Y. enterocolitica 0:8 WA-C(pYV), resulting in WA-C(pYVLcrV(K42Q)), which turned out to be less virulent for C57BL/6 mice than the parental strain. This difference in virulence was not observed in TLR2(-/-) or IL-10(-/-) mice, proving that LcrV contributes to virulence by TLR2-mediated IL-10 induction. LcrV is a defined bacterial virulence factor shown to target the TLR system for evasion of the host's immune response.
引用
收藏
页码:16049 / 16054
页数:6
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