The Sun Health Research Institute Brain Donation Program: Description and experience, 1987-2007

被引：208

作者：

Beach, Thomas G. ^{[1
]}

Sue, Lucia I. ^{[1
]}

Walker, Douglas G. ^{[1
]}

Roher, Alex E. ^{[1
]}

Lue, LihFen ^{[1
]}

Vedders, Linda ^{[1
]}

Connor, Donald J. ^{[1
]}

Sabbagh, Marwan N. ^{[1
]}

Rogers, Joseph ^{[1
]}

机构：

[1] Sun Hlth Res Inst, Sun City, AZ 85351 USA

来源：

CELL AND TISSUE BANKING | 2008年 / 9卷 / 03期

关键词：

brain banking; neuropathology; postmortem; autopsy; tissue banking; RNA; pH; cerebrospinal fluid; postmortem interval;

D O I：

10.1007/s10561-008-9067-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The Brain Donation Program at Sun Health Research Institute has been in continual operation since 1987, with over 1000 brains banked. The population studied primarily resides in the retirement communities of northwest metropolitan Phoenix, Arizona. The Institute is affiliated with Sun Health, a nonprofit community-owned and operated health care provider. Subjects are enrolled prospectively to allow standardized clinical assessments during life. Funding comes primarily from competitive grants. The Program has made short postmortem brain retrieval a priority, with a 2.75-h median postmortem interval for the entire collection. This maximizes the utility of the resource for molecular studies; frozen tissue from approximately 82% of all cases is suitable for RNA studies. Studies performed in-house have shown that, even with very short postmortem intervals, increasing delays in brain retrieval adversely affect RNA integrity and that cerebrospinal fluid pH increases with postmortem interval but does not predict tissue viability.

引用

页码：229 / 245

页数：17

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[1] NITRIC-OXIDE (NO) IS INVOLVED IN INCREASED CEREBRAL CORTICAL BLOOD-FLOW FOLLOWING STIMULATION OF THE NUCLEUS BASALIS OF MEYNERT IN ANESTHETIZED RATS [J].