Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondria

被引:199
作者
Kispal, G
Sipos, K
Lange, H
Fekete, Z
Bedekovics, T
Janáky, T
Bassler, J
Netz, DJA
Balk, J
Rotte, C
Lill, R
机构
[1] Univ Marburg, Inst Zytobiol & Zytopathol, D-35033 Marburg, Germany
[2] Univ Med Sch Pecs, Inst Biochem, Pecs, Hungary
[3] Univ Szeged, Fac Med, Dept Med Chem, Szeged, Hungary
[4] Biochem Zentrum Heidelberg, Heidelberg, Germany
关键词
ABC transporter; iron homeostasis; iron-sulphur cluster; nuclear export; protein translation;
D O I
10.1038/sj.emboj.7600541
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mitochondria perform a central function in the biogenesis of cellular iron - sulphur (Fe/S) proteins. It is unknown to date why this biosynthetic pathway is indispensable for life, the more so as no essential mitochondrial Fe/S proteins are known. Here, we show that the soluble ATP-binding cassette (ABC) protein Rli1p carries N-terminal Fe/S clusters that require the mitochondrial and cytosolic Fe/ S protein biogenesis machineries for assembly. Mutations in critical cysteine residues of Rli1p abolish association with Fe/ S clusters and lead to loss of cell viability. Hence, the essential character of Fe/ S clusters in Rli1p explains the indispensable character of mitochondria in eukaryotes. We further report that Rli1p is associated with ribosomes and with Hcr1p, a protein involved in rRNA processing and translation initiation. Depletion of Rli1p causes a nuclear export defect of the small and large ribosomal subunits and subsequently a translational arrest. Thus, ribosome biogenesis and function are intimately linked to the crucial role of mitochondria in the maturation of the essential Fe/ S protein Rli1p.
引用
收藏
页码:589 / 598
页数:10
相关论文
共 51 条
[31]   Protein import into mitochondria [J].
Neupert, W .
ANNUAL REVIEW OF BIOCHEMISTRY, 1997, 66 :863-917
[32]   Versatility of the mitochondrial protein import machinery [J].
Pfanner, N ;
Geissler, A .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2001, 2 (05) :339-349
[33]   The tandem affinity purification (TAP) method:: A general procedure of protein complex purification [J].
Puig, O ;
Caspary, F ;
Rigaut, G ;
Rutz, B ;
Bouveret, E ;
Bragado-Nilsson, E ;
Wilm, M ;
Séraphin, B .
METHODS, 2001, 24 (03) :218-229
[34]   A novel eukaryotic factor for cytosolic Fe-S cluster assembly [J].
Roy, A ;
Solodovnikova, N ;
Nicholson, T ;
Antholine, W ;
Walden, WE .
EMBO JOURNAL, 2003, 22 (18) :4826-4835
[35]  
Sambrook J, 2001, MOL CLONING LAB MANU, DOI DOI 10.1016/0003-2697(90)90595-Z
[36]  
Scheffler I.E., 1999, MITOCHONDRIA
[37]  
Schuller C, 2003, ABC PROTEINS BACTERI, P279
[38]  
SHERMAN F, 1991, METHOD ENZYMOL, V194, P3
[39]   Maturation of cytosolic iron-sulfur proteins requires glutathione [J].
Sipos, K ;
Lange, H ;
Fekete, Z ;
Ullmann, P ;
Lill, R ;
Kispal, G .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (30) :26944-26949
[40]   A MOSSBAUER SPECTROSCOPIC INVESTIGATION OF THE REDOX BEHAVIOR OF THE MOLYBDENUM-IRON PROTEIN FROM KLEBSIELLA-PNEUMONIAE NITROGENASE - MECHANISTIC AND STRUCTURAL IMPLICATIONS [J].
SMITH, BE ;
ODONNELL, MJ ;
LANG, G ;
SPARTALIAN, K .
BIOCHEMICAL JOURNAL, 1980, 191 (02) :449-455