Plasma miR-136 can be used to screen patients with knee osteoarthritis from healthy controls by targeting IL-17

被引：27

作者：

Wan, Lei ^{[1
]}

Zhao, Qiang ^{[2
]}

Niu, Gang ^{[3
]}

Xiang, Tai ^{[4
]}

Ding, Cuilei ^{[4
]}

Wang, Shijie ^{[5
]}

机构：

[1] Rizhao Peoples Hosp, Dept Joint Surg, Rizhao 276800, Shandong, Peoples R China

[2] Second Peoples Hosp Rizhao City, Dept Bone Surg, Rizhao 276800, Shandong, Peoples R China

[3] Rizhao Peoples Hosp, Surg Dept, Rizhao 276800, Shandong, Peoples R China

[4] Rizhao Peoples Hosp, Dept Surg, Rizhao 276800, Shandong, Peoples R China

[5] Rizhao Peoples Hosp, Dept Orthoped, 126 Taian Rd, Rizhao 276800, Shandong, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2018年 / 16卷 / 04期

关键词：

miR-136; knee osteoarthritis; interleukin-17; biomarker; ARTHROSCOPIC SURGERY; INTERLEUKIN-17; ASSOCIATION; EXPRESSION; SERUM;

D O I：

10.3892/etm.2018.6625

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

The current study mainly evaluated the plasma level of microRNA (miR)-136 in knee osteoarthritis (KOA) patients and determined if miR-136 could be used as a potential biomarker to screen KOA patients from healthy controls. It was demonstrated that plasma miR-136 was significantly decreased in the plasma of KOA patients. Moreover, the reduction in plasma miR-136 negatively correlated with the severity of KOA. Additionally, the increase in the serum interleukin (IL)-17 level positively correlated with the severity of KOA. More importantly, dual luciferase assays and western blot assays indicated that IL-17 was a target gene of miR-136. Further analysis showed that plasma miR-136 could be used as a biomarker to screen KOA patients from healthy controls. In summary, for the first time, the present study revealed that through targeting IL-17, decreased plasma miR-136 levels could be used as a potential biomarker to screen KOA patients from healthy controls.

引用

页码：3419 / 3424

页数：6

共 30 条

[1]

DEVELOPMENT OF CRITERIA FOR THE CLASSIFICATION AND REPORTING OF OSTEOARTHRITIS - CLASSIFICATION OF OSTEOARTHRITIS OF THE KNEE [J].

ALTMAN, R ;

ASCH, E ;

BLOCH, D ;

BOLE, G ;

BORENSTEIN, D ;

BRANDT, K ;

CHRISTY, W ;

COOKE, TD ;

GREENWALD, R ;

HOCHBERG, M ;

HOWELL, D ;

KAPLAN, D ;

KOOPMAN, W ;

LONGLEY, S ;

MANKIN, H ;

MCSHANE, DJ ;

MEDSGER, T ;

MEENAN, R ;

MIKKELSEN, W ;

MOSKOWITZ, R ;

MURPHY, W ;

ROTHSCHILD, B ;

SEGAL, M ;

SOKOLOFF, L ;

WOLFE, F .

ARTHRITIS AND RHEUMATISM, 1986, 29 (08) :1039-1049

[2]

Mast cells are the main interleukin 17-positive cells in anticitrullinated protein antibody-positive and -negative rheumatoid arthritis and osteoarthritis synovium (Retraction of vol 12, R150, 2011) [J].

Suurmond, Jolien ;

Dorjee, Annemarie L. ;

Boon, Mariette R. ;

Knol, Edward F. ;

Huizinga, Tom W. J. ;

Toes, Rene E. M. ;

Schuerwegh, Annemie J. M. .

ARTHRITIS RESEARCH & THERAPY, 2015, 17

[3]

Interleukin-17 up-regulation of nitric oxide production in human osteoarthritis cartilage [J].

Attur, MG ;

Patel, RN ;

Abramson, SB ;

Amin, AR .

ARTHRITIS AND RHEUMATISM, 1997, 40 (06) :1050-1053

[4]

Altered Expression of Circulating MicroRNA in Plasma of Patients with Primary Osteoarthritis and In Silico Analysis of Their Pathways [J].

Borgonio Cuadra, Veronica M. ;

Celia Gonzalez-Huerta, Norma ;

Romero-Cordoba, Sandra ;

Hidalgo-Miranda, Alfredo ;

Miranda-Duarte, Antonio .

PLOS ONE, 2014, 9 (06)

[5]

Association between severity of knee osteoarthritis and serum and synovial fluid interleukin 17 concentrations [J].

Chen, Biao ;

Deng, Yu ;

Tan, Yang ;

Qin, Jun ;

Chen, Liao-Bin .

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 2014, 42 (01) :138-144

[6]

Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients [J].

Deligne, C. ;

Casulli, S. ;

Pigenet, A. ;

Bougault, C. ;

Campillo-Gimenez, L. ;

Nourissat, G. ;

Berenbaum, F. ;

Elbim, C. ;

Houard, X. .

OSTEOARTHRITIS AND CARTILAGE, 2015, 23 (11) :1843-1852

[7]

Evaniew AL, 2017, WORLD J ORTHOP, V8, P187, DOI 10.5312/wjo.v8.i2.187

[8]

MicroRNA-9 regulates the development of knee osteoarthritis through the NF-kappaB1 pathway in chondrocytes [J].

Gu, Ronghe ;

Liu, Ning ;

Luo, Simin ;

Huang, Weiguo ;

Zha, Zhengang ;

Yang, Jie .

MEDICINE, 2016, 95 (36)

[9]

Molecular Mechanism of MiR-136-5p Targeting NF-κB/A20 in the IL-17-Mediated Inflammatory Response after Spinal Cord Injury [J].

He, Jichen ;

Zhao, Jinmin ;

Peng, Xiaoming ;

Shi, Xiongzhi ;

Zong, Shaohui ;

Zeng, Gaofeng .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2017, 44 (03) :1224-1241

[10]

Contribution of interleukin 17 to human cartilage degradation and synovial inflammation in osteoarthritis [J].

Honorati, MC ;

Bovara, M ;

Cattini, L ;

Piacentini, A ;

Facchini, A .

OSTEOARTHRITIS AND CARTILAGE, 2002, 10 (10) :799-807

← 1 2 3 →