Preparation of highly deuterated zeaxanthin, lycopene, and β-carotene from fully deuterated mevalonate using engineered Escherichia coli

被引：8

作者：

Eguchi, T ^{[1
]}

Dekishima, Y

Aizawa, H

Tamegai, H

Kakinuma, K

Misawa, N

Kuzuyama, T

Seto, H

机构：

[1] Tokyo Inst Technol, Dept Chem & Mat Sci, Meguro Ku, Tokyo 1528551, Japan

[2] Tokyo Inst Technol, Dept Chem, Meguro Ku, Tokyo 1528551, Japan

[3] Maine Biotechnol Inst, Kamaishi 0260001, Japan

[4] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan

[5] Tokyo Univ Agr, Dept Appl Biol & Chem, Setagaya Ku, Tokyo 1568562, Japan

来源：

TETRAHEDRON | 2005年 / 61卷 / 08期

关键词：

carotenoids; biosynthesis; isotope labeling; engineered Escherichia coli;

D O I：

10.1016/j.tet.2004.12.056

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Carotenoids are important natural pigments produced by various microorganisms and plants. Specific deuterium-labeling of these compounds is invaluable in biochemical and physiochemical research. In this paper, preparation of highly deuterated zeaxanthin, lycopene, and beta-carotene using engineered Escherichia coli with fully deuterated mevalonate is described. Also described are physico-chemical properties of the obtained deuterated carotenoids. (C) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2027 / 2035

页数：9

共 41 条

[1] Metabolic engineering of the terpenoid biosynthetic pathway of Escherichia coli for production of the carotenoids β-carotene and zeaxanthin [J].