Preparation of highly deuterated zeaxanthin, lycopene, and β-carotene from fully deuterated mevalonate using engineered Escherichia coli

被引：8

作者：

Eguchi, T ^{[1
]}

Dekishima, Y

Aizawa, H

Tamegai, H

Kakinuma, K

Misawa, N

Kuzuyama, T

Seto, H

机构：

[1] Tokyo Inst Technol, Dept Chem & Mat Sci, Meguro Ku, Tokyo 1528551, Japan

[2] Tokyo Inst Technol, Dept Chem, Meguro Ku, Tokyo 1528551, Japan

[3] Maine Biotechnol Inst, Kamaishi 0260001, Japan

[4] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan

[5] Tokyo Univ Agr, Dept Appl Biol & Chem, Setagaya Ku, Tokyo 1568562, Japan

来源：

TETRAHEDRON | 2005年 / 61卷 / 08期

关键词：

carotenoids; biosynthesis; isotope labeling; engineered Escherichia coli;

D O I：

10.1016/j.tet.2004.12.056

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Carotenoids are important natural pigments produced by various microorganisms and plants. Specific deuterium-labeling of these compounds is invaluable in biochemical and physiochemical research. In this paper, preparation of highly deuterated zeaxanthin, lycopene, and beta-carotene using engineered Escherichia coli with fully deuterated mevalonate is described. Also described are physico-chemical properties of the obtained deuterated carotenoids. (C) 2005 Elsevier Ltd. All rights reserved.

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页码：2027 / 2035

页数：9

相关论文

共 41 条

[21] Biosynthesis of isoprenoids in Escherichia coli:: stereochemistry of the reaction catalyzed by isopentenyl diphosphate:dimethylallyl diphosphate isomerase [J].

Leyes, AE ;

Baker, JA ;

Hahn, FM ;

Poulter, CD .

CHEMICAL COMMUNICATIONS, 1999, (08) :717-718

[22] Biosynthesis of isoprenoids in Escherichia coli:: Stereochemistry of the reaction catalyzed by farnesyl diphosphate synthase [J].

Leyes, AE ;

Baker, JA ;

Poulter, CD .

ORGANIC LETTERS, 1999, 1 (07) :1071-1073

[23] Metabolic engineering of carotenoid accumulation in Escherichia coli by modulation of the isoprenoid precursor pool with expression of deoxyxylulose phosphate synthase [J].

Matthews, PD ;

Wurtzel, ET .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2000, 53 (04) :396-400

[24] CANTHAXANTHIN BIOSYNTHESIS BY THE CONVERSION OF METHYLENE TO KETO GROUPS IN A HYDROCARBON BETA-CAROTENE BY A SINGLE-GENE [J].

MISAWA, N ;

KAJIWARA, S ;

KONDO, K ;

YOKOYAMA, A ;

SATOMI, Y ;

SAITO, T ;

MIKI, W ;

OHTANI, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 209 (03) :867-876

[25] STRUCTURE AND FUNCTIONAL-ANALYSIS OF A MARINE BACTERIAL CAROTENOID BIOSYNTHESIS GENE-CLUSTER AND ASTAXANTHIN BIOSYNTHETIC-PATHWAY PROPOSED AT THE GENE LEVEL [J].

MISAWA, N ;

SATOMI, Y ;

KONDO, K ;

YOKOYAMA, A ;

KAJIWARA, S ;

SAITO, T ;

OHTANI, T ;

MIKI, W .

JOURNAL OF BACTERIOLOGY, 1995, 177 (22) :6575-6584

[26]

MISHINA IM, 1993, BIOTEKHNOLOGIYA, P13

[27] Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli [J].

Rodríguez-Concepción, M ;

Campos, N ;

Lois, LM ;

Maldonado, C ;

Hoeffler, JF ;

Grosdemange-Billiard, C ;

Rohmer, M ;

Boronat, A .

FEBS LETTERS, 2000, 473 (03) :328-332

[28]

Rohmer, 1999, COMPREHENSIVE NATURA, V2, P45

[29] The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants [J].

Rohmer, M .

NATURAL PRODUCT REPORTS, 1999, 16 (05) :565-574

[30]

Sambrook J, 2001, MOL CLONING LAB MANU, DOI DOI 10.1016/0003-2697(90)90595-Z

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