High-throughput single-molecule optofluidic analysis

被引：68

作者：

Kim, Soohong ^{[1
]}

Streets, Aaron M. ^{[2
]}

Lin, Ron R. ^{[1
]}

Quake, Stephen R. ^{[2
,3
,4
]}

Weiss, Shimon ^{[1
,5
,6
]}

Majumdar, Devdoot S. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90024 USA

[2] Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[4] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

[5] Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90024 USA

[6] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90024 USA

来源：

NATURE METHODS | 2011年 / 8卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

RESONANCE ENERGY-TRANSFER; RNA-POLYMERASE; DNA; KINETICS; SCALE; MIXER;

D O I：

10.1038/NMETH.1569

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

引用

页码：242 / U83

页数：6

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