Derivation of multipotent mesenchymal precursors from human embryonic stem cells

被引：329

作者：

Barberi, T

Willis, LM

Socci, ND

Studer, L ^{[1
]}

机构：

[1] Sloan Kettering Inst, Lab Stem Cell & Tumor Biol, Div Neurosurg, New York, NY 10021 USA

[2] Sloan Kettering Inst, Dev Biol Program, New York, NY USA

[3] Sloan Kettering Inst, Computat Biol Ctr, New York, NY USA

来源：

PLOS MEDICINE | 2005年 / 2卷 / 06期

关键词：

D O I：

10.1371/journal.pmed.0020161

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background Human embryonic stem cells provide access to the earliest stages of human development and may serve as a source of specialized cells for regenerative medicine. Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic stem cells into tissue-restricted precursors. Methods and Findings Here, we present culture conditions for the derivation of unlimited numbers of pure mesenchymal precursors from human embryonic stem cells and demonstrate multilineage differentiation into fat, cartilage, bone, and skeletal muscle cells. Conclusion Our findings will help to elucidate the mechanism of mesoderm specification during embryonic stem cell differentiation and provide a platform to efficiently generate specialized human mesenchymal cell types for future clinical applications.

引用

收藏

页码：554 / 560

页数：7

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