Ganoderma lucidum polysaccharides in human monocytic leukemia cells:: from gene expression to network construction

被引：60

作者：

Cheng, Kun-Chieh ^{[1
,2
]}

Huang, Hsuan-Cheng ^{[3
]}

Chen, Jenn-Han ^{[4
]}

Hsu, Jia-Wei ^{[5
]}

Cheng, Hsu-Chieh ^{[1
]}

Ou, Chern-Han ^{[1
,6
]}

Yang, Wen-Bin ^{[7
,8
]}

Chen, Shui-Tein ^{[7
,8
,9
]}

Wong, Chi-Huey ^{[7
,8
,9
,10
,11
]}

Juan, Hsueh-Fen ^{[1
,5
,12
,13
]}

机构：

[1] Natl Taiwan Univ, Dept Life Sci, Taipei 106, Taiwan

[2] Natl Taiwan Univ, Inst Biotechnol, Taipei 106, Taiwan

[3] Natl Yang Ming Univ, Inst Biomed Informat, Taipei 112, Taiwan

[4] Natl Def Univ, Natl Def Med Ctr, Sch Dent, Taipei 114, Taiwan

[5] Natl Taiwan Univ, Inst Mol & Cellular Biol, Taipei 106, Taiwan

[6] Natl Taiwan Univ, Dept Elect Engn, Taipei 10764, Taiwan

[7] Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan

[8] Acad Sinica, Genom Res Ctr, Taipei 115, Taiwan

[9] Natl Taiwan Univ, Inst Biochem Sci, Taipei 106, Taiwan

[10] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[11] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[12] Natl Taiwan Univ, Inst Biomed Elect & Bioinformat, Taipei 106, Taiwan

[13] Natl Taiwan Univ, Ctr Syst Biol & Bioinformat, Taipei 106, Taiwan

来源：

BMC GENOMICS | 2007年 / 8卷 / 1期

关键词：

D O I：

10.1186/1471-2164-8-411

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Background: Ganoderma lucidum has been widely used as a herbal medicine for promoting health and longevity in China and other Asian countries. Polysaccharide extracts from Ganoderma lucidum have been reported to exhibit immuno-modulating and anti-tumor activities. In previous studies, F3, the active component of the polysaccharide extract, was found to activate various cytokines such as IL-1, IL-6, IL-12, and TNF-alpha. This gave rise to our investigation on how F3 stimulates immuno-modulating or anti-tumor effects in human leukemia THP-1 cells. Results: Here, we integrated time-course DNA microarray analysis, quantitative PCR assays, and bioinformatics methods to study the F3-induced effects in THP-1 cells. Significantly disturbed pathways induced by F3 were identified with statistical analysis on microarray data. The apoptosis induction through the DR3 and DR4/5 death receptors was found to be one of the most significant pathways and play a key role in THP-1 cells after F3 treatment. Based on time-course gene expression measurements of the identified pathway, we reconstructed a plausible regulatory network of the involved genes using reverse-engineering computational approach. Conclusion: Our results showed that F3 may induce death receptor ligands to initiate signaling via receptor oligomerization, recruitment of specialized adaptor proteins and activation of caspase cascades.

引用

页数：17

共 62 条

[1]

Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[2]

Apoptosis control by death and decoy receptors [J].

Ashkenazi, A ;

Dixit, VM .

CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (02) :255-260

[3]

Death receptors: Signaling and modulation [J].

Ashkenazi, A ;

Dixit, VM .

SCIENCE, 1998, 281 (5381) :1305-1308

[4]

Oncogenic pathway signatures in human cancers as a guide to targeted therapies [J].

Bild, AH ;

Yao, G ;

Chang, JT ;

Wang, QL ;

Potti, A ;

Chasse, D ;

Joshi, MB ;

Harpole, D ;

Lancaster, JM ;

Berchuck, A ;

Olson, JA ;

Marks, JR ;

Dressman, HK ;

West, M ;

Nevins, JR .

NATURE, 2006, 439 (7074) :353-357

[5]

Death to the bad guys: Targeting cancer via Apo2L/TRAIL [J].

Bouralexis, S ;

Findlay, DM ;

Evdokiou, A .

APOPTOSIS, 2005, 10 (01) :35-51

[6]

Tumor necrosis factor-α-induced protein 3 as a putative regulator of nuclear factor-κB-mediated resistance to O6-alkylating agents in human glioblastomas [J].

Bredel, M ;

Bredel, C ;

Juric, D ;

Duran, GE ;

Yu, RX ;

Harsh, GR ;

Vogel, H ;

Recht, LD ;

Scheck, AC ;

Sikic, BI .

JOURNAL OF CLINICAL ONCOLOGY, 2006, 24 (02) :274-287

[7]

Studies on the immuno-modulating and anti-tumor activities of Ganoderma lucidum (Reishi) polysaccharides [J].

Chen, HS ;

Tsai, YF ;

Lin, S ;

Lin, CC ;

Khoo, KH ;

Lin, CH ;

Wong, CH .

BIOORGANIC & MEDICINAL CHEMISTRY, 2004, 12 (21) :5595-5601

[8]

Chui CH, 2006, ONCOL REP, V16, P1313

[9]

ArrayXPath: mapping and visualizing microarray gene-expression data with integrated biological pathway resources using Scalable Vector Graphics [J].

Chung, HJ ;

Kim, M ;

Park, CH ;

Kim, J ;

Kim, JH .

NUCLEIC ACIDS RESEARCH, 2004, 32 :W460-W464

[10]

Conservation of gene order: a fingerprint of proteins that physically interact [J].

Dandekar, T ;

Snel, B ;

Huynen, M ;

Bork, P .

TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (09) :324-328

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