Resolution of cis-trans ambiguities between HLA-DRB1 alleles using single-strand conformation polymorphisms and sequencing

被引：12

作者：

Paul, P ^{[1
]}

Thomas, D ^{[1
]}

Kawczak, P ^{[1
]}

Good, D ^{[1
]}

Cook, DJ ^{[1
]}

Ball, EJ ^{[1
]}

机构：

[1] Cleveland Clin Fdn, Allogen Labs, Cleveland, OH 44195 USA

来源：

TISSUE ANTIGENS | 2001年 / 57卷 / 04期

关键词：

cis/trans ambiguity; DNA-based histocompatibility typing; HLA-DRB1; PCR-SSCP;

D O I：

10.1034/j.1399-0039.2001.057004300.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

DNA-based typing of HLA alleles occasionally results in the inability to assign a specific allele because of ambiguity in associating two or more polymorphisms to the same or to alternate homologs (cis/trans ambiguity). Since most individuals are heterozygous at a given HLA locus, the highest level of confidence in definition is obtained when the alleles are tested in isolation. By using single-strand conformation polymorphisms (SSCP) to separate heterozygous HLA-DRB1 alleles, followed by sequencing of separated conformers, we have achieved resolution of previously ambiguous assignments without the need for additional probes or primers.

引用

页码：300 / 307

页数：8

共 30 条

[1] High resolution HLA class I typing by reference strand mediated conformation analysis (RSCA) [J].

Argüello, JR ;

Little, AM ;

Bohan, E ;

Goldman, JM ;

Marsh, SGE ;

Madrigal, JA .

TISSUE ANTIGENS, 1998, 52 (01) :57-66

[2] Mutation detection and typing of polymorphic loci through double-strand conformation analysis [J].

Argüello, JR ;

Little, AM ;

Pay, AL ;

Gallardo, D ;

Rojas, I ;

Marsh, SGE ;

Goldman, JM ;

Madrigal, JA .

NATURE GENETICS, 1998, 18 (02) :192-194

[3] A novel method for simultaneous high resolution identification of HLA-A, HLA-B, and HLA-Cw alleles [J].

Arguello, R ;

Avakian, H ;

Goldman, JM ;

Madrigal, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (20) :10961-10965

[4] STRUCTURE OF THE HUMAN CLASS-I HISTOCOMPATIBILITY ANTIGEN, HLA-A2 [J].

BJORKMAN, PJ ;

SAPER, MA ;

SAMRAOUI, B ;

BENNETT, WS ;

STROMINGER, JL ;

WILEY, DC .

NATURE, 1987, 329 (6139) :506-512

[5] COMPREHENSIVE, SEROLOGICALLY EQUIVALENT DNA TYPING FOR HLA-B BY PCR USING SEQUENCE-SPECIFIC PRIMERS (PCR-SSP) [J].

BUNCE, M ;

FANNING, GC ;

WELSH, KI .

TISSUE ANTIGENS, 1995, 45 (02) :81-90

[6] Nucleotide sequences of MHC class I introns 1, 2, and 3 in humans and intron 2 in nonhuman primates [J].

Cereb, N ;

Kong, Y ;

Lee, S ;

Maye, P ;

Yang, SY .

TISSUE ANTIGENS, 1996, 47 (06) :498-511

[7] HLA-DQB1 AND DQA1 MATCHING BY AMBIENT-TEMPERATURE PCR-SSCP [J].

CLAY, TM ;

CULPAN, D ;

PURSALL, MC ;

BRADLEY, BA ;

BIDWELL, JL .

EUROPEAN JOURNAL OF IMMUNOGENETICS, 1995, 22 (06) :467-478

[8] HLA-DR-TYPING, DQ-TYPING AND DP-TYPING USING PCR AMPLIFICATION AND IMMOBILIZED PROBES [J].

ERLICH, H ;

BUGAWAN, T ;

BEGOVICH, AB ;

SCHARF, S ;

GRIFFITH, R ;

SAIKI, R ;

HIGUCHI, R ;

WALSH, PS .

EUROPEAN JOURNAL OF IMMUNOGENETICS, 1991, 18 (1-2) :33-55

[9]

FERNANDEZVINA MA, 1997, GENETIC DIVERSITY HL, P596

[10] OPTIMIZATION OF THE SINGLE-STRAND CONFORMATION POLYMORPHISM (SSCP) TECHNIQUE FOR DETECTION OF POINT MUTATIONS [J].

GLAVAC, D ;

DEAN, M .

HUMAN MUTATION, 1993, 2 (05) :404-414

← 1 2 3 →