Separation and simultaneous determination of nalidixic acid, hydroxynalidixic acid and carboxynalidixic acid in serum and urine by micellar electrokinetic capillary chromatography

被引:15
作者
Pérez-Ruiz, T [1 ]
Martínez-Lozano, C [1 ]
Sanz, A [1 ]
Bravo, E [1 ]
机构
[1] Univ Murcia, Fac Chem, Dept Analyt Chem, E-30071 Murcia, Spain
来源
JOURNAL OF CHROMATOGRAPHY B | 1999年 / 724卷 / 02期
关键词
nalidixic acid; hydroxynalidixic acid; carboxynalidixic acid;
D O I
10.1016/S0378-4347(98)00564-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Separation in capillary electrophoresis is governed by various factors, including buffer type, buffer concentration, pH, temperature, voltage and micelles. Through proper adjustment of these parameters, nalidixic acid and its two major metabolites, 7-hydroxynalidixic and 7-carboxynalidixic, could be separated by micellar electrokinetic capillary chromatography using an electrophoretic electrolyte consisting of 50 mM berate buffer (pH 9) containing 25 mM sodium dodecyl sulphate and 10% acetonitrile. A linear relationship between concentration and peak area for each compound was obtained in the concentration range 0.15-100 mu g ml(-1), with a correlation coefficient greater than 0.999 and detection limits in the 0.2-0.7 ng ml(-1) range. Intra- and inter-day precision values of about 0.8-1.2% RSD (n = 11) and 1.3-2.0% RSD (n = 30), respectively, were obtained. The method has been applied to the analysis of nalidixic acid and its two major metabolites in serum and urine with limits of sensitivity lower than 0.8 ng ml(-1). (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:319 / 324
页数:6
相关论文
共 15 条
[1]  
*AM HOSP FORM SERV, 1998, DRUG INF 98
[2]   DETERMINATION OF NALIDIXIC-ACID AND ITS 2 MAJOR METABOLITES IN HUMAN-PLASMA AND URINE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
CUISINAUD, G ;
FERRY, N ;
SECCIA, M ;
BERNARD, N ;
SASSARD, J .
JOURNAL OF CHROMATOGRAPHY, 1980, 181 (3-4) :399-406
[3]  
Dondi G., 1996, B CHIM FARM, V105, P491
[4]   SPECTROFLUOROMETRIC DETERMINATION OF NALIDIXIC-ACID BASED ON HOST-GUEST COMPLEXATION WITH GAMMA-CYCLODEXTRIN [J].
DURANMERAS, I ;
DELAPENA, AM ;
SALINAS, F ;
CACERES, IR .
ANALYST, 1994, 119 (06) :1215-1219
[5]   CLARIFICATION OF THE LIMIT OF DETECTION IN CHROMATOGRAPHY [J].
FOLEY, JP ;
DORSEY, JG .
CHROMATOGRAPHIA, 1984, 18 (09) :503-511
[6]   POLAROGRAPHIC-BEHAVIOR AND DETERMINATION OF NORFLOXACIN IN TABLETS [J].
JABER, AMY ;
LOUNICI, A .
ANALYTICA CHIMICA ACTA, 1994, 291 (1-2) :53-64
[7]   ABSORPTION EXCRETION + METABOLISM OF NEW ANTIBACTERIAL AGENT NALIDIXIC ACID [J].
MCCHESNEY, EW ;
LESHER, GY ;
CRAIN, AVR ;
ROSI, D ;
FROELICH, EJ .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1964, 6 (03) :292-&
[8]  
ROSEBOOM H, 1979, J CHROMATOGR, V163, P92, DOI 10.1016/S0378-4347(00)81174-3
[9]   NALIDIXIC ACID AND HYDROXYNALIDIXIC ACID ANALYSIS IN HUMAN PLASMA AND URINE BY LIQUID CHROMATOGRAPHY [J].
SHARGEL, L ;
KOSS, RF ;
CRAIN, AVR ;
BOYLE, VJ .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1973, 62 (09) :1452-1454
[10]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF NALIDIXIC-ACID AND HYDROXYNALIDIXIC ACID IN PLASMA WITH A DYNAMIC ANION-EXCHANGE SYSTEM [J].
SOREL, RHA ;
HULSHOFF, A ;
SNELLEMAN, C .
JOURNAL OF CHROMATOGRAPHY, 1980, 221 (01) :129-137