HIV-1 reverse transcriptase and integrase enzymes physically interact and inhibit each other

被引:55
作者
Tasara, T
Maga, G
Hottiger, MO
Hübscher, U
机构
[1] Univ Zurich Irchel, Inst Vet Biochem & Mol Biol, CH-8057 Zurich, Switzerland
[2] CNR, Ist Genet Biochim & Evoluzionist, I-27100 Pavia, Italy
关键词
reverse transcription; integration; preintegration complex;
D O I
10.1016/S0014-5793(01)02945-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ordered molecular interactions and structural changes must take place within the human immunodeficiency virus type 1 (HIV-1) preintegration complex at various stages for successful viral replication. We demonstrate both physical and biochemical interactions between HIV-1 reverse transcriptase and integrase enzymes. This interaction may have implications on the in vivo functions of the two enzyme; within the HIV-1 replication complex. It may be one of the various molecular interactions, which facilitate efficient HIV-1 replication within the target cells. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:39 / 44
页数:6
相关论文
共 25 条
  • [11] Directed integration of viral DNA mediated by fusion proteins consisting of human immunodeficiency virus type I integrase and Escherichia coli LexA protein
    Goulaouic, H
    Chow, SA
    [J]. JOURNAL OF VIROLOGY, 1996, 70 (01) : 37 - 46
  • [12] GRANDGENETT DP, 1994, PROTEIN SCI, V3, P888
  • [13] HOTTIGER M, 1994, J BIOL CHEM, V269, P986
  • [14] HOTTIGER M, 1995, NUCLEIC ACIDS RES, V23, P736
  • [15] Role for human immunodeficiency virus type 1 Tat protein in suppression of viral reverse transcriptase activity during late stages of viral replication
    Kameoka, M
    Rong, LW
    Götte, M
    Liang, C
    Russell, RS
    Wainberg, MA
    [J]. JOURNAL OF VIROLOGY, 2001, 75 (06) : 2675 - 2683
  • [16] Role of matrix in an early postentry step in the human immunodeficiency virus type 1 life cycle
    Kiernan, RE
    Ono, A
    Englund, G
    Freed, EO
    [J]. JOURNAL OF VIROLOGY, 1998, 72 (05) : 4116 - 4126
  • [17] Involvement of HIV-1 nucleocapsid protein in the recruitment of reverse transcriptase into nucleoprotein complexes formed in vitro
    Lener, D
    Tanchou, V
    Roques, BP
    Le Grice, SFJ
    Darlix, JL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (50) : 33781 - 33786
  • [18] Human immunodeficiency virus type 1 preintegration complexes: Studies of organization and composition
    Miller, MD
    Farnet, CM
    Bushman, FD
    [J]. JOURNAL OF VIROLOGY, 1997, 71 (07) : 5382 - 5390
  • [19] Essential amino acids for substrate binding and catalysis of human flap endonuclease 1
    Shen, BH
    Nolan, JP
    Sklar, LA
    Park, MS
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (16) : 9173 - 9176
  • [20] GENETIC-ANALYSIS OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE PROTEIN
    SHIN, CG
    TADDEO, B
    HASELTINE, WA
    FARNET, CM
    [J]. JOURNAL OF VIROLOGY, 1994, 68 (03) : 1633 - 1642