Determination of punicalagin isomers in pomegranate husk

被引:91
作者
Lu, Jingjing [1 ]
Ding, Ke [2 ]
Yuan, Qipeng [1 ]
机构
[1] Beijing Univ Chem Technol, Key Lab Bioproc Beijing, Beijing 100029, Peoples R China
[2] China Agr Univ, Dept Food Sci, Beijing 102206, Peoples R China
基金
中国国家自然科学基金;
关键词
column liquid chromatography; pomegranate husk; punincalagin;
D O I
10.1365/s10337-008-0699-y
中图分类号
Q5 [生物化学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
Punicalagins are the main ingredients of phenolic compounds in pomegranate (Punica granatum L.) husk. A simple and accurate method for punicalagin analysis based on ethanol extraction and RP-LC using linear gradient of methanol in 0.1% TFA solution was established. The feasibility of this procedure was tested by analyzing the punicalagin level both in fresh pomegranate husk collected from different provinces in China and dried husk from a drugstore. The content of each isomer and total content of punicalagins in husk were determined. The mean value of punicalagins content in pomegranate husk was 82.4 mg g(-1). The highest content of punicalagins was found in a variety of husks from Shanxi province, while the lowest content was found in the husk from the drugstore in Guangdong province.
引用
收藏
页码:303 / 306
页数:4
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