Characterization of hitchhiker, a transposon insertion frequently associated with baculovirus FP mutants derived upon passage in the TN-368 cell line

被引：10

作者：

Bauser, CA ^{[1
]}

Elick, TA ^{[1
]}

Fraser, MJ ^{[1
]}

机构：

[1] UNIV NOTRE DAME,DEPT BIOL SCI,NOTRE DAME,IN 46556

来源：

VIROLOGY | 1996年 / 216卷 / 01期

关键词：

D O I：

10.1006/viro.1996.0053

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We characterize a new Lepidopteran transposon associated with FP mutations of baculoviruses. This transposon, designated hitchhiker, is 579 bp long with 39-bp imperfect inverted terminal repeals. hitchhiker inserts with extreme specificity for a single trinucleotide target site, TTA, within the 25K gene. This transposon is the most frequently identified insertion in serial passage baculovirus mutants isolated from TN-368 cells. (C) 1996 Academic Press, Inc.

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页码：235 / 237

页数：3

相关论文

共 17 条

[1] SEQUENCE COMPARISON OF CELLULAR AND VIRAL COPIES OF HOST-CELL DNA INSERTIONS FOUND IN AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS [J].

BEAMES, B ;

SUMMERS, MD .

VIROLOGY, 1990, 174 (02) :354-363

[2] LOCATION AND NUCLEOTIDE-SEQUENCE OF THE 25K PROTEIN MISSING FROM BACULOVIRUS FEW POLYHEDRA (FP) MUTANTS [J].

BEAMES, B ;

SUMMERS, MD .

VIROLOGY, 1989, 168 (02) :344-353

[3] COMPARISONS OF HOST-CELL DNA INSERTIONS AND ALTERED TRANSCRIPTION AT THE SITE OF INSERTIONS IN FEW POLYHEDRA BACULOVIRUS MUTANTS [J].

BEAMES, B ;

SUMMERS, MD .

VIROLOGY, 1988, 162 (01) :206-220

[4] IDENTIFICATION AND NUCLEOTIDE-SEQUENCE OF THE REGIONS OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS GENOME CARRYING INSERTION ELEMENTS DERIVED FROM SPODOPTERA-FRUGIPERDA [J].

CARSTENS, EB .

VIROLOGY, 1987, 161 (01) :8-17

[5] TRANSPOSON MUTAGENESIS OF BACULOVIRUSES - ANALYSIS OF TRICHOPLUSIA-NI TRANSPOSON IFP2 INSERTIONS WITHIN THE FP-LOCUS OF NUCLEAR POLYHEDROSIS VIRUSES [J].

CARY, LC ;

GOEBEL, M ;

CORSARO, BG ;

WANG, HG ;

ROSEN, E ;

FRASER, MJ .

VIROLOGY, 1989, 172 (01) :156-169

[6] Transposable elements and DNA transposition in eukaryotes [J].

Finnegan, D. J. .

CURRENT OPINION IN CELL BIOLOGY, 1990, 2 (03) :471-477

[7]

Fraser M. J., 1987, Biotechnology in invertebrate pathology and cell culture., P265

[8] ACQUISITION OF HOST-CELL DNA-SEQUENCES BY BACULOVIRUSES - RELATIONSHIP BETWEEN HOST DNA INSERTIONS AND FP MUTANTS OF AUTOGRAPHA-CALIFORNICA AND GALLERIA-MELLONELLA NUCLEAR POLYHEDROSIS VIRUSES [J].

FRASER, MJ ;

SMITH, GE ;

SUMMERS, MD .

JOURNAL OF VIROLOGY, 1983, 47 (02) :287-300

[9] TRANSPOSON-MEDIATED MUTAGENESIS OF BACULOVIRUSES - TRANSPOSON SHUTTLING AND IMPLICATIONS FOR SPECIATION [J].

FRASER, MJ .

ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA, 1986, 79 (05) :773-783

[10] TRANSPOSON-MEDIATED MUTAGENESIS OF A BACULOVIRUS [J].

FRASER, MJ ;

BRUSCA, JS ;

SMITH, GE ;

SUMMERS, MD .

VIROLOGY, 1985, 145 (02) :356-361