Characterization of hitchhiker, a transposon insertion frequently associated with baculovirus FP mutants derived upon passage in the TN-368 cell line

被引:10
作者
Bauser, CA [1 ]
Elick, TA [1 ]
Fraser, MJ [1 ]
机构
[1] UNIV NOTRE DAME,DEPT BIOL SCI,NOTRE DAME,IN 46556
关键词
D O I
10.1006/viro.1996.0053
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We characterize a new Lepidopteran transposon associated with FP mutations of baculoviruses. This transposon, designated hitchhiker, is 579 bp long with 39-bp imperfect inverted terminal repeals. hitchhiker inserts with extreme specificity for a single trinucleotide target site, TTA, within the 25K gene. This transposon is the most frequently identified insertion in serial passage baculovirus mutants isolated from TN-368 cells. (C) 1996 Academic Press, Inc.
引用
收藏
页码:235 / 237
页数:3
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