Novel characteristics of community-acquired methicillin-resistant Staphylococcus aureus strains belonging to multilocus sequence type 59 in Taiwan

被引:137
作者
Takano, Tomomi [1 ]
Higuchi, Wataru [1 ]
Otsuka, Taketo [1 ]
Baranovich, Tatiana [1 ]
Enany, Shymaa [1 ]
Saito, Kohei [1 ]
Isobe, Hirokazu [1 ]
Dohmae, Soshi [1 ]
Ozaki, Kyoko [1 ]
Takano, Nfisao [1 ]
Iwao, Yasuhisa [1 ]
Shibuya, Michiko [1 ]
Okubo, Takeshi [1 ]
Yabe, Shizuka [1 ]
Shi, Da [1 ]
Reva, Ivan [1 ]
Teng, Lee-Jene [2 ,3 ]
Yamamoto, Tatsuo [1 ]
机构
[1] Niigata Univ, Grad Sch Med & Dent Sci, Dept Infect Dis Control & Int Med, Div Bacteriol, Niigata, Japan
[2] Natl Taiwan Univ, Coll Med, Taipei, Taiwan
[3] Natl Taiwan Univ Hosp, Taipei, Taiwan
关键词
D O I
10.1128/AAC.01001-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strains, which often produce Panton-Valentine leucocidin (PVL), are increasingly noted worldwide. In this study, we examined 42 MRSA strains (25 PVL-positive [PVL+] strains and 17 PVL-negative [PVL-] strains) isolated in Taiwan for their molecular characteristics. The PVL+ MRSA strains included CA-MRSA strains with multilocus sequence type (ST) 59 (major PVL+ MRSA in Taiwan), its variants, and worldwide CA-MRSA ST30 strains. The PVL(-)MRSA strains included the pandemic Hungarian MRSA ST239 strain, the Hungarian MRSA ST239 variant, MRSA ST59 (largely hospital-acquired MRSA strains) and its variants, the pandemic New York/Japan MRSA ST5 strain (Japanese type), and the MRSA ST8 strain. The major PVL+ CA-MRSA ST59 strain possessed a tetracycline resistance-conferring (tetK positive) penicillinase plasmid and a drug resistance gene cluster (a possible composite transposon) for multidrug resistance. Moreover, it carried a novel staphylococcal cassette chromosome mec (SCCmec) with two distinct ccrC genes (ccrC2-C8). This SCCmec (previously named SCCmec type V-T) was tentatively designated SCCmec type VII. Sequencing of the PVL genes revealed the polymorphisms, and the PVL+ CA-MRSA ST59 strain possessed the ST59-specific PVL gene sequence. The data suggest that a significant amount of clonal spread is occurring in Taiwan and that the major PVL+ CA-MRSA ST59(Taiwan) strain exhibits unique genetic characteristics, such as a novel SCCmec type and an ST59-specific PVL gene sequence.
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页码:837 / 845
页数:9
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