Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus

被引：94

作者：

Jothikumar, N

Cromeans, TL

Sobsey, MD

Robertson, BH

机构：

[1] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Viral Hepatitis, Dept Parasit Dis, Atlanta, GA 30341 USA

[2] Univ N Carolina, Dept Environm Sci & Engn, Chapel Hill, NC 27599 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 06期

关键词：

D O I：

10.1128/AEM.71.6.3359-3363.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Primers and a TaqMan probe for the 5'-untransiated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.

引用

页码：3359 / 3363

页数：5

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