Quantitative determination of ginsenosides by high-performance liquid chromatography-tandem mass spectrometry

被引:93
作者
Ji, QC
Harkey, MR [1 ]
Henderson, GL
Gershwin, ME
Stern, JS
Hackman, RM
机构
[1] Univ Calif Davis, Sch Med, Dept Med Pharmacol & Toxicol, Davis, CA 95616 USA
[2] ThermoQuest Corp, San Jose, CA 95134 USA
[3] Univ Calif Davis, Ctr Complementary & Alternat Med Res Asthma & All, Davis, CA 95616 USA
[4] Univ Calif Davis, Div Rheumatol Allergy & Clin Immunol, Dept Med, Davis, CA 95616 USA
[5] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA
[6] Univ Calif Davis, Div Clin Nutr, Dept Internal Med, Davis, CA 95616 USA
关键词
high performance liquid chromatography-mass spectrometry; ginsenosides; Panax ginseng; Panax quinquefolius; ginseng;
D O I
10.1002/pca.593
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An HPLC-MS/MS method was developed for the quantitative determination of ginsenosides, which are the marker compounds for herbal products containing Panax ginseng (Korean or Chinese ginseng) and P. quinquefolius (American ginseng). Samples were extracted with BondElut((R)) C-18 HF extraction columns and the concentrations of seven major ginsenosides (Rb-1, Rb-2, Re, Rd, Re, Rf, and Rg(1)) were determined by reversed-phase HPLC-MS/MS employing a quadrupole-ion trap mass spectrometer. Both positive and negative electrospray ionisation techniques were evaluated. Positive ionisation spectra of these compounds gave strong sodium adduct molecular and sodium adduct dimer ions. Negative ionisation yielded the molecular ion primarily and was, therefore, used for analysis: quantitative determination was based on the most abundant product ions for each ginsenoside. The method was used to extract and analyse commercial samples of P. ginseng and P. quinquefolius. Copyright (C) 2001 John Wiley & Sons, Ltd.
引用
收藏
页码:320 / 326
页数:7
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