Consolidation of particle beds and packing of chromatographic columns

被引：104

作者：

Guiochon, G ^{[1
]}

Farkas, T ^{[1
]}

GuanSajonz, H ^{[1
]}

Koh, JH ^{[1
]}

Sarker, M ^{[1
]}

Stanley, BJ ^{[1
]}

Yun, T ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,DIV CHEM & ANALYT SCI,OAK RIDGE,TN 37831

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 762卷 / 1-2期

关键词：

column packing; stationary phases; LC; packing methods; reviews;

D O I：

10.1016/S0021-9673(96)00642-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of complementary evidence reviewed here demonstrates that the packing of chromatographic columns is heterogeneous. It is denser and less well organized close to the column wall than in the center of the column. The mechanism of particle consolidation under stress explains the origin of this phenomenon and provides a fundamental justification to what is known in column chromatography as ''the wall effect''.

引用

页码：83 / 88

页数：6

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