PSP toxins from Aphanizomenon flos-aquae (cyanobacteria) collected in the Crestuma-Lever reservoir (Douro river, northern Portugal)

被引:80
作者
Ferreira, FMB
Soler, JMF
Fidalgo, ML
Fernández-Vila, P
机构
[1] Inst Nacl Saude Dr Ricardo Jorge, Parasitol Lab, P-4000 Oporto, Portugal
[2] CSIC, Inst Invest Marinas, Vigo 36208, Spain
[3] Ctr Galego Control Calidade Med Marino Xunta Gali, Pontevedra, Spain
关键词
PSP toxins; RP-HPLC; Aphanizomenon; cyanobacteria;
D O I
10.1016/S0041-0101(00)00114-8
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The presence of paralytic shellfish poisoning (PSP) toxins in cultures of Aphanizomenon flos-aquae, isolated from the Crestuma-Lever reservoir, was found by reversed phase high performance liquid chromatography employing two isocratic elution systems for the separation of PSP toxins. With the first isocratic elution protocol, the presence of apolar toxins as saxitoxin, decarbamoyl saxitoxin and neosaxitoxin not detected. On the other hand, GTX4, GTX1 and GTX3 as well as Cs toxins were present either in the Aphanizomenon flos-aquae cells collected directly from the bloom or in the other toxic isolates priorly cultivated in laboratory conditions. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:757 / 761
页数:5
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