Characterization of an Arabidopsis thaliana mutant that has a defect in ABA accumulation:: ABA-dependent and ABA-independent accumulation of free amino acids during dehydration

被引:134
作者
Nambara, E
Kawaide, H
Kamiya, Y
Naito, S [1 ]
机构
[1] Hokkaido Univ, Fac Agr, Dept Appl Biosci, Sapporo, Hokkaido 0608589, Japan
[2] RIKEN, Inst Phys & Chem Res, Frontier Res Program, Lab Plant Hormone Funct, Wako, Saitama 3510198, Japan
关键词
ABA; Arabidopsis; branched-chain amino acids; dehydration;
D O I
10.1093/oxfordjournals.pcp.a029444
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In an attempt to elucidate the physiological role of ABA in seed dormancy and the adaptive response to dehydration, we isolated an ABA-deficient mutant of Arabidopsis thaliana (L.) Heynh. which germinated in the presence of a gibberellin biosynthetic inhibitor. Genetic analysis showed this mutation is a new allele of a recently reported locus aba2, and therefore has been designated aba2-2. The levels of endogenous ABA in fresh and dehydrated tissues of the aba2-2 mutant were highly reduced compared to those of wild-type plants. As a consequence, aba2-2 plants wilt and produce seeds with reduced dormancy. Dark germinated seedlings of the aba2-2 mutant showed true leaves, which were not observed in those of the wild type, indicating that aba2-2 embryos grew precociously during seed maturation. In the dehydrated tissues of the wildtype plants, the levels of free proline, isoleucine and leucine were elevated to a content approximately 100-fold higher than those in fresh tissues. In contrast to the wild-type plants, dehydration-induced accumulation of proline was highly suppressed in the aba2-2 mutant plants while that of leucine and isoleucine accumulated. Furthermore, exogenous application of ABA to wild-type plants promoted accumulation of free proline, but not leucine nor isoleucine. These results suggest that dehydration-induced accumulation of free leucine and isoleucine is achieved independent of ABA.
引用
收藏
页码:853 / 858
页数:6
相关论文
共 33 条
[1]   ASSIGNMENT OF 30 MICROSATELLITE LOCI TO THE LINKAGE MAP OF ARABIDOPSIS [J].
BELL, CJ ;
ECKER, JR .
GENOMICS, 1994, 19 (01) :137-144
[2]   WATER DEFICIT-INDUCED CHANGES IN ABSCISIC-ACID, GROWTH, POLYSOMES, AND TRANSLATABLE RNA IN SOYBEAN HYPOCOTYLS [J].
BENSEN, RJ ;
BOYER, JS ;
MULLET, JE .
PLANT PHYSIOLOGY, 1988, 88 (02) :289-294
[3]   Herbicide safener-inducible gene expression in Arabidopsis thaliana [J].
De Veylder, L ;
Van Montagu, M ;
Inze, D .
PLANT AND CELL PHYSIOLOGY, 1997, 38 (05) :568-577
[4]   PROLINE BIOSYNTHESIS AND OSMOREGULATION IN PLANTS [J].
DELAUNEY, AJ ;
VERMA, DPS .
PLANT JOURNAL, 1993, 4 (02) :215-223
[5]   ABSCISIC-ACID-DEFICIENT MUTANTS AT THE ABA GENE LOCUS OF ARABIDOPSIS-THALIANA ARE IMPAIRED IN THE EPOXIDATION OF ZEAXANTHIN [J].
DUCKHAM, SC ;
LINFORTH, RST ;
TAYLOR, IB .
PLANT CELL AND ENVIRONMENT, 1991, 14 (06) :601-606
[6]  
GAWRONSKA H, 1995, PLANT CELL PHYSIOL, V36, P1361
[7]   MOLECULAR ANALYSIS AND SPATIAL EXPRESSION PATTERN OF A LOW-TEMPERATURE-SPECIFIC BARLEY GENE, BLT101 [J].
GODDARD, NJ ;
DUNN, MA ;
ZHANG, L ;
WHITE, AJ ;
JACK, PL ;
HUGHES, MA .
PLANT MOLECULAR BIOLOGY, 1993, 23 (04) :871-879
[8]   ES13, A STRESS-INDUCED GENE FROM LOPHOPYRUM-ELONGATUM [J].
GULICK, PJ ;
SHEN, W ;
AN, HY .
PLANT PHYSIOLOGY, 1994, 104 (02) :799-800
[9]   EVIDENCE FOR CROSS-PATHWAY REGULATION OF METABOLIC GENE-EXPRESSION IN PLANTS [J].
GUYER, D ;
PATTON, D ;
WARD, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (11) :4997-5000
[10]   METABOLIC RESPONSES OF MESOPHYTES TO PLANT WATER DEFICITS [J].
HANSON, AD ;
HITZ, WD .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1982, 33 :163-203