Superior field performance of waxy corn engineered using CRISPR-Cas9

被引：151

作者：

Gao, Huirong ^{[1
]}

Gadlage, Mark J. ^{[1
]}

Lafitte, H. Renee ^{[1
,2
]}

Lenderts, Brian ^{[1
]}

Yang, Meizhu ^{[1
]}

Schroder, Megan ^{[1
]}

Farrell, Jeffry ^{[1
]}

Snopek, Kay ^{[1
]}

Peterson, Dave ^{[1
]}

Feigenbutz, Lanie ^{[1
]}

Jones, Spencer ^{[1
]}

St Clair, Grace ^{[1
]}

Rahe, Melissa ^{[1
]}

Sanyour-Doyel, Nathalie ^{[1
]}

Peng, Chenna ^{[1
]}

Wang, Lijuan ^{[1
]}

Young, Joshua K. ^{[1
]}

Beatty, Mary ^{[1
]}

Dahlke, Brian ^{[1
]}

Hazebroek, Jan ^{[1
]}

Greene, Thomas W. ^{[1
]}

Cigan, A. Mark ^{[1
,3
]}

Chilcoat, N. Doane ^{[1
]}

Meeley, R. Bob ^{[1
]}

机构：

[1] Corteva Agrisci, Res & Dev, Johnston, IA 50131 USA

[2] Bill & Melinda Gates Fdn, Seattle, WA USA

[3] Genus Res, De Forest, WI USA

来源：

NATURE BIOTECHNOLOGY | 2020年 / 38卷 / 05期

关键词：

MAIZE; STARCH;

D O I：

10.1038/s41587-020-0444-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We created waxy corn hybrids by CRISPR-Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than conventional trait introgression using backcrossing and marker-assisted selection. Field trials at 25 locations showed that CRISPR-waxy hybrids were agronomically superior to introgressed hybrids, producing on average 5.5 bushels per acre higher yield. Gene-edited waxy corn lines have higher yields in field trials than hybrids produced by traditional trait introgression.

引用

收藏

页码：579 / +

页数：7

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