Isolation of Δ9-THCA-A from hemp and analytical aspects concerning the determination of Δ9-THC in cannabis products

被引：165

作者：

Dussy, FE ^{[1
]}

Hamberg, C ^{[1
]}

Luginbühl, M ^{[1
]}

Schwerzmann, T ^{[1
]}

Briellmann, TA ^{[1
]}

机构：

[1] Univ Basel, Inst Legal Med, CH-4056 Basel, Switzerland

来源：

FORENSIC SCIENCE INTERNATIONAL | 2005年 / 149卷 / 01期

关键词：

Delta(9)-THCA-A; Delta(9)-THC; total-Delta(9)-THC; decarboxylation; isolation;

D O I：

10.1016/j.forsciint.2004.05.015

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A simple procedure based on a common silica gel column chromatography for the isolation of Delta(9)-tetrahydrocannabinolic acid A (Delta(9)-THCA-A) from hemp in a multi-milligram scale is presented. Further, the decarboxylation reaction of Delta(9)-THCA-A to the toxicologically active Delta(9)-tetrahydrocannabinol (Delta(9)-THC) at different analytical and under-smoking conditions is investigated. Maximal conversion in an optimised analytical equipment yields about 70% Delta(9)-THC. In the simulation of the smoking process, only about 30% of the spiked substance could be recovered as Delta(9)-THC. (c) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：3 / 10

页数：8

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