The use of fungal in vitro systems for studying translational regulation

被引：35

作者：

Wu, Cheng ^{[1
]}

Amrani, Nadia

Jacobson, Altan

Sachs, Matthew S.

机构：

[1] Oregon Hlth & Sci Univ, OGI Sch Sci & Engn, Dept Environm & Biomol Syst, Beaverton, OR USA

[2] Univ Massachusetts, Sch Med, Dept Mol Genet & Microbiol, Worcester, MA USA

[3] Oregon Hlth & Sci Univ, Sch Med, Dept Mol Microbiol & Immunol, Portland, OR 97201 USA

来源：

TRANSLATION INITIATION: EXTRACT SYSTEMS AND MOLECULAR GENETICS | 2007年 / 429卷

关键词：

D O I：

10.1016/S0076-6879(07)29010-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of cell-free systems enables biochemical determination of factors and mechanisms contributing to translational processes. The preparation and use of cell-free translation systems from the fungi Saccharomyces cerevisiae and Neurospora crassa are described. Examples provided illustrate the use of these systems, in conjunction with luciferase assays, [(35)S]Met incorporation, and primer-extension inhibition (toeprint) analyses, to assess the translational effects of upstream open reading frames and premature termination codons.

引用

页码：203 / 225

页数：23

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