Construction of a gorilla fosmid library and its PCR screening system

被引：35

作者：

Kim, CG

Fujiyama, A

Saitou, N ^{[1
]}

机构：

[1] Natl Inst Genet, Div Populat Genet, Mishima, Shizuoka 4118540, Japan

[2] Natl Inst Informat, Chiyoda Ku, Tokyo 1018430, Japan

来源：

GENOMICS | 2003年 / 82卷 / 05期

关键词：

gorilla; ape; hominoid; fosmid library; comparative genomics; HOX; evolution;

D O I：

10.1016/S0888-7543(03)00174-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A gorilla fosmid library of 261,120 independent clones was constructed and characterized. The fosmid vector is similar to the cosmid in average insert size of ca. 40 kb but contains the F factor for replication, and it is more resistant to recombination. This clone library represents about 3.7 times coverage of the gorilla genome. A simple screening system by PCR was established, and we successfully found 9 clones that cover the entire Hox A gene cluster of the gorilla genome. This gorilla fosmid DNA library is a useful resource for comparative genomics of human and apes. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：571 / 574

页数：4

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