Characterization of a specific antibody to the rat angiotensin II AT1 receptor

被引：66

作者：

Giles, ME ^{[1
]}

Fernley, RT ^{[1
]}

Nakamura, Y ^{[1
]}

Moeller, I ^{[1
]}

Aldred, GP ^{[1
]}

Ferraro, T ^{[1
]}

Penschow, JD ^{[1
]}

McKinley, MJ ^{[1
]}

Oldfield, BJ ^{[1
]}

机构：

[1] Univ Melbourne, Howard Florey Inst Expt Physiol & Med, Parkville, Vic 3052, Australia

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1999年 / 47卷 / 04期

关键词：

angiotensin II; AT(1) receptor; lamina terminalis;

D O I：

10.1177/002215549904700409

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT(1) receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. These included (a) the ultrastructural localization of immunogold-labeled receptor on the surfaces of zona glomerulosa cells in the adrenal cortex, (b) the specific labeling of Chinese hamster ovarian (CHO) cells transfected with AT(1) receptors, (c) the identification of a specific band on Western blots, (d) the immunocytochemical co-localization of angiotensin receptors on neurons in the lamina terminalis of the brain shown to be responsive to circulating angiotensin II, as shown by the expression of c-fos, and (e) the correlation between the expression of the mRNA of the AT(1) receptor and AT(1) receptor immunoreactivity.

引用

页码：507 / 515

页数：9

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