Large sequence variation at two microsatellite loci among zoonotic (genotype C) isolates of Cryptosporidium parvum

被引：51

作者：

Cacciò, S ^{[1
]}

Spano, F ^{[1
]}

Pozio, E ^{[1
]}

机构：

[1] Ist Super Sanita, Parasitol Lab, I-00161 Rome, Italy

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2001年 / 31卷 / 10期

关键词：

Cryptosporidium parvum; genotyping; microsatellites; DNA sequence analysis;

D O I：

10.1016/S0020-7519(01)00233-8

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The genetic polymorphism among 57 Cryptosporidium parvum isolates belonging to genotype 'C' was studied by PCR amplification and the sequencing of two microsatellite loci (ML1 and ML2). A comparative analysis of DNA sequences showed the presence of three (ML1-238, ML1-226, and ML1-220) and seven (ML2-231, ML2-229, ML2-227, ML2-213. ML2-193, ML2-191, and ML2-187) different alleles at these two loci. Alleles differed by expansions/contractions of the microsatellite repeats that generated length polymorphisms. Some alleles were found to be associated with infections of all examined hosts (calf, kid, lamb, and human), whereas others were either associated with a single host, or were geographically restricted. When considering the information from both loci, some preferential associations between alleles are apparent. These data confirm the utility of microsatellite markers for the molecular identification of C. parvum, which is of particular relevance in the investigation of the source of infection of outbreaks and single cases, its well as for genetic studies. (C) 2001 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：1082 / 1086

页数：5

共 22 条

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