Effects of aging on DNA-binding activity of the E47 transcription factor in splenic B cells

被引：10

作者：

Frasca, D ^{[1
]}

Nguyen, D ^{[1
]}

Riley, RL ^{[1
]}

Blomberg, BB ^{[1
]}

机构：

[1] Univ Miami, Sch Med, Dept Microbiol & Immunol, Miami, FL 33136 USA

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 2004年 / 125卷 / 02期

关键词：

aging; splenic B cells; E47 transcription factor;

D O I：

10.1016/j.mad.2003.11.004

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and lambda5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：111 / 112

页数：2

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