Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes

被引:54
作者
Pimkova, Kristyna [1 ]
Bockova, Marketa [2 ]
Hegnerova, Katerina [2 ]
Suttnar, Jiri [1 ]
Cermak, Jaroslav [1 ]
Homola, Jiri [2 ]
Dyr, Jan E. [1 ]
机构
[1] Inst Hematol & Blood Transfus, CR-12820 Prague, Czech Republic
[2] Inst Photon & Elect, Prague 18251, Czech Republic
关键词
Surface plasmon resonance; Myelodysplastic syndromes; Vascular endothelial growth factor; Protein markers; ENDOTHELIAL GROWTH-FACTOR; ACUTE MYELOID-LEUKEMIA; FACTOR RECEPTOR-1; BLOOD-PLASMA; VEGF; ANGIOGENESIS; NEUROPILIN-1; PROGRESSION; EXPRESSION; PREDICTS;
D O I
10.1007/s00216-011-5395-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.
引用
收藏
页码:381 / 387
页数:7
相关论文
共 25 条
[1]  
[Anonymous], MYELODYSPLASTIC SYND
[2]   Measurement of free and complexed soluble vascular endothelial growth factor receptor, Flt-I, in fluid samples: development and application of two new immunoassays [J].
Belgore, FM ;
Blann, AD ;
Lip, GY .
CLINICAL SCIENCE, 2001, 100 (05) :567-575
[3]   Characterization of vascular endothelial cell growth factor interactions with the kinase insert domain-containing receptor tyrosine kinase - A real time kinetic study [J].
Cunningham, SA ;
Tran, TM ;
Arrate, MP ;
Brock, TA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (26) :18421-18427
[4]  
Fuh G, 2000, J BIOL CHEM, V275, P26690
[5]   Systems Biology of Vascular Endothelial Growth Factors [J].
Gabhann, Feilim Mac ;
Popel, Aleksander S. .
MICROCIRCULATION, 2008, 15 (08) :715-738
[6]   An SPR biosensor for the detection of microcystins in drinking water [J].
Herranz, Sonia ;
Bockova, Marketa ;
Dolores Marazuela, Maria ;
Homola, Jiri ;
Cruz Moreno-Bondi, Maria .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 398 (06) :2625-2634
[7]   Surface plasmon resonance sensors for detection of chemical and biological species [J].
Homola, Jiri .
CHEMICAL REVIEWS, 2008, 108 (02) :462-493
[8]   Soluble vascular endothelial growth factor receptor 1, and not receptor 2, is an independent prognostic factor in acute myeloid leukemia and myelodysplastic syndromes [J].
Hu, QL ;
Dey, AL ;
Yang, Y ;
Shen, Y ;
Jilani, IB ;
Estey, EH ;
Kantarjian, HM ;
Giles, FJ ;
Albitar, M .
CANCER, 2004, 100 (09) :1884-1891
[9]   Expression of soluble VEGF receptor 2 and characterization of its binding by surface plasmon resonance [J].
Huang, XM ;
Gottstein, C ;
Brekken, RA ;
Thorpe, PE .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 252 (03) :643-648
[10]  
Jeevaratnam K, 2008, HYPERTENS PREGNANCY, V27, P657