Isolation of γ-oryzanol through calcium ion induced precipitation of anionic micellar aggregates

被引：28

作者：

Das, PK ^{[1
]}

Chaudhuri, A ^{[1
]}

Kaimal, TNB ^{[1
]}

Bhalerao, UT ^{[1
]}

机构：

[1] Indian Inst Chem Technol, Div Lipid Sci & Technol, Hyderabad 500007, Andhra Pradesh, India

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1998年 / 46卷 / 08期

关键词：

gamma-oryzanol; ferulic acid esters; CAD esters; HPLC; LSIMS;

D O I：

10.1021/jf9710802

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A simple method for the isolation of gamma-oryzanol from rice bran acid oil has been developed. The basis of the present isolation protocol is calcium ion induced precipitation or anionic micellar aggregates. Rice bran acid oil, a byproduct of rice bran oil processing industries, is subjected to conventional vacuum distillation to remove free fatty acids. Aqueous alkaline hydrolysis of the resultant residue, upon dilution with water, produces anionic micellar aggregates containing solubilized gamma-oryzanol. Addition of calcium ions to this aqueous micellar aggregate induces instant coprecipitation of the calcium salts of the fatty acids and the aggregate-associated gamma-oryzanol. The dried precipitate is extracted with ethyl acetate. Ethyl acetate is evaporated from the extract, and gamma-oryzanol is purified from the residue by silica gel column chromatography. Experimental details for the isolation and characterization procedure are described. The present method will give ready access to the physiologically beneficial and value-added pharmaceutical product gamma-oryzanol.

引用

页码：3073 / 3080

页数：8