Characterization and applications of reversed-phase column selectivity based on the hydrophobic-subtraction model

被引：70

作者：

Marchand, D. H. ^{[1
]}

Snyder, L. R. ^{[2
]}

Dolan, J. W. ^{[2
]}

机构：

[1] Univ Wisconsin, River Falls, WI 54022 USA

[2] LC Resources Inc, Amity, OR 97101 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1191卷 / 1-2期

关键词：

column selectivity; peak tailing; orthogonal separation; column stability; hydrophobic-subtraction model;

D O I：

10.1016/j.chroma.2007.10.079

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A total of 371 reversed-phase columns have now been characterized in terms of selectivity, based on five solute-column interactions (the hydrophobic-subtraction model). The present study illustrates the use of these data for interpreting peak-tailing and column stability. New insights are also provided concerning column selectivity as a function of ligand and silica type, and the selection of columns for orthogonal separations is re-examined. Some suggestions for the quality control of reversed-phase columns during manufacture are offered. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：2 / 20

页数：19

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