Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments

被引：664

作者：

Van Heukelem, L ^{[1
]}

Thomas, CS ^{[1
]}

机构：

[1] Univ Maryland, Horn Point Lab, Ctr Environm Sci, Cambridge, MD 21613 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 910卷 / 01期

关键词：

chromatography modeling software; stationary phases; LC; phytoplankton pigments; carotenoids; chlorophylls;

D O I：

10.1016/S0378-4347(00)00603-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We used chromatography modeling software to assist in HPLC method development, with the goal of enhancing separations through the exclusive use of gradient time and column temperature. We surveyed nine stationary phases for their utility in pigment purification and natural sample analysis. For purification, a complex algal matrix was separated on an efficient monomeric column, from which partially purified fractions were collected and purified on polymeric columns that exaggerated resolution between pigments of interest. Additionally, we feature an HPLC method that is simple, fast, demonstrates excellent transferability and is ideal for quantitative analysis of pigments in dilute natural water samples. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：31 / 49

页数：19

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