A rapid method for determination of Staphylococcus aureus based on milk coagulation by using a series piezoelectric quartz crystal sensor

被引:15
作者
Bao, LL [1 ]
Tan, HW [1 ]
Duan, Q [1 ]
Su, XL [1 ]
Wei, WZ [1 ]
机构
[1] Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Peoples R China
基金
中国国家自然科学基金;
关键词
SPQC sensor; S-aureus; milk coagulation;
D O I
10.1016/S0003-2670(98)00208-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A series piezoelectric quartz crystal (SPQC) sensor was utilized to determine the number of bacteria based on the coagulation of milk in which Staphylococcus aureus (S. aureus) had grown. Compared with the method based on a PQC coated with a thin film, earlier it had the advantage that no dilution of the medium was needed and cease of the oscillation can be avoided throughout the experiment. Moreover, it was rapid, the turning-point time (TT) for quantitative detection which was the time at which the frequency begins to return after a drop in response curve was easy to determine and there was a good relation between TT and logarithm of the initial concentration of S. aureus in the range of 2.4 x 10(2)-2.4 x 10(5) cells ml(-1). (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:139 / 145
页数:7
相关论文
共 10 条
[1]   Determination of microorganisms with a quartz crystal microbalance sensor [J].
Bao, LL ;
Deng, L ;
Nie, LH ;
Yao, SZ ;
Wei, WZ .
ANALYTICA CHIMICA ACTA, 1996, 319 (1-2) :97-101
[2]   RAPID DETECTION OF ESCHERICHIA COLIFORM USING A SERIES ELECTRODE PIEZOELECTRIC CRYSTAL SENSOR [J].
HE, FJ ;
ZHU, WH ;
GENG, Q ;
NIE, LH ;
YAO, SZ .
ANALYTICAL LETTERS, 1994, 27 (04) :655-669
[3]  
ISHIMORI Y, 1981, J APPL ENV MICROBIOL, V30, P483
[4]   NETWORK ANALYSIS METHOD APPLIED TO LIQUID-PHASE ACOUSTIC-WAVE SENSORS [J].
KIPLING, AL ;
THOMPSON, M .
ANALYTICAL CHEMISTRY, 1990, 62 (14) :1514-1519
[5]   A GOAT-ANTI-HUMAN IGG MODIFIED PIEZOIMMUNOSENSOR FOR STAPHYLOCOCCUS-AUREUS DETECTION [J].
LE, D ;
HE, FJ ;
JIANG, TJ ;
NIE, LH ;
YAO, SZ .
JOURNAL OF MICROBIOLOGICAL METHODS, 1995, 23 (02) :229-234
[6]  
MILLER R, 1989, J I BREWING, V95, P316
[7]  
MURAMATSU H, 1989, ANAL LETT, V22, P2155
[8]   PIEZOELECTRIC IMMUNO SENSOR FOR THE DETECTION OF CANDIDA-ALBICANS MICROBES [J].
MURAMATSU, H ;
KAJIWARA, K ;
TAMIYA, E ;
KARUBE, I .
ANALYTICA CHIMICA ACTA, 1986, 188 :257-261
[9]  
PETTIFER GL, 1980, J APPL ENV MICROBIOL, V29, P29
[10]  
SHEN DZ, 1994, THESIS HUNAN U