Macroporous chitosan/carboxymethylclellulose blend membranes and their application for lysozyme adsorption

被引:60
作者
Chen, X [1 ]
Liu, JH [1 ]
Feng, ZC [1 ]
Shao, ZZ [1 ]
机构
[1] Fudan Univ, Key Lab Mol Engn Polymers, Dept Macromol Sci, Shanghai 200433, Peoples R China
关键词
biopolymers; chromatography; ion exchangers; polyelectrolytes; proteins;
D O I
10.1002/app.21552
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
The adsorption of lysozyme was investigated with novel macroporous chitosan (CS)/carboxymethyl/cellulose (CMC) blend membranes. The CS/CMG blend membranes were prepared by a simple solution-blending method with glutaraldehyde as a crosslinking agent for CS and with silica particles as porogens. The CS/CMC blend membranes were insoluble in aqueous media when the CMC concentration in the membranes did not exceed 30 mol %. The protein adsorption on these membranes from aqueous solutions containing different amounts of lysozyme at different pHs was investigated in batch systems. The results showed that the lysozyme adsorption capacity had a maximum at pH 9.2, and this indicated that the CS/CMC blend membranes could act as cation-exchange membranes. Moreover, the blend membranes showed the best adsorption properties for lysozyme when the CMC concentration was 20 mol %. In addition, the lysozyme adsorption capacity of the blend membranes increased with an increase in the initial lysozyme concentration and the adsorption temperature. The maximum adsorption capacity of the macroporous CS/CMC blend membranes was as high as 240 mg/g (170 mg/mL), and more than 95% of the adsorbed lysozyme was desorbed in a pH buffer at 11.8. The blend membranes also demonstrated good reusability after several adsorption-desorption cycles. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:1267 / 1274
页数:8
相关论文
共 27 条
[1]   Purification of a plant peroxidase using reversibly soluble ion-exchange polymer [J].
Aruna, N ;
Lali, A .
PROCESS BIOCHEMISTRY, 2001, 37 (04) :431-437
[2]   ON THE FIXED CHARGE CONCENTRATION AND THE WATER ELECTROOSMOTIC TRANSPORT IN A CELLULOSE-ACETATE MEMBRANE [J].
BARRAGAN, VM ;
RUEDA, C ;
RUIZBAUZA, C .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1995, 172 (02) :361-367
[3]   Procion Green H-4G immobilized on a new IPN hydrogel membrane composed of poly(2-hydroxyethylmethacrylate)/chitosan: preparation and its application to the adsorption of lysozyme [J].
Bayramoglu, G ;
Arica, MY .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2002, 202 (01) :41-52
[4]  
Charcosset C, 1998, J CHEM TECHNOL BIOT, V71, P95, DOI 10.1002/(SICI)1097-4660(199802)71:2<95::AID-JCTB823>3.0.CO
[5]  
2-J
[6]  
Chen X, 1997, J APPL POLYM SCI, V65, P2257, DOI 10.1002/(SICI)1097-4628(19970912)65:11<2257::AID-APP23>3.0.CO
[7]  
2-Z
[8]   Controlled mixed-mode interaction chromatography on membrane adsorbers [J].
Freitag, R ;
Splitt, H ;
Reif, OW .
JOURNAL OF CHROMATOGRAPHY A, 1996, 728 (1-2) :129-137
[9]   Protein separation using membrane chromatography: opportunities and challenges [J].
Ghosh, R .
JOURNAL OF CHROMATOGRAPHY A, 2002, 952 (1-2) :13-27
[10]   Procion Green H-E4BD-immobilized porous poly(hydroxyethylmethacrylate) ion-exchange membrane:: preparation and application to lysozyme adsorption [J].
Kaçar, Y ;
Arica, MY .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2001, 22 (03) :227-236