Drug resistance evolution of a Mycobacterium tuberculosis strain from a noncompliant patient

被引:31
作者
Meacci, F
Orrù, G
Iona, E
Giannoni, F
Piersimoni, C
Pozzi, G
Fattorini, L
Oggioni, MR
机构
[1] Univ Siena, Policlin Le Scotte, LAMMB, Dipartimento Biol Mol, I-53100 Siena, Italy
[2] Univ Cagliari, Dipartimento Sci Odontostomatol, I-09124 Cagliari, Italy
[3] Ist Super Sanita, Dipartimento Malattie Infett Parassitaire & Immun, I-00161 Rome, Italy
[4] Osped Riuniti Bergamo, Dipartimento Microbiol Clin, Ancona, Italy
关键词
D O I
10.1128/JCM.43.7.3114-3120.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The emergence and spread of multidrug-resistant (MDR) Mycobacterium tuberculosis (MT) represents a worldwide health care problem because of the difficulty in treating these infections. Development of drug resistance in MT arises mainly by mutation of chromosomal genes. To investigate the evolution of a NIT population during a long-lasting infection, the phenotypic and genotypic changes in the drug resistance of 10 sequential MT isolates from a noncompliant chronically infected patient were investigated. During more than 12 years of active disease, a MDR population developed; molecular typing showed one single parental strain that infected the patient and persisted throughout the disease. Molecular analysis of the drug resistance-related genes revealed that discrete subpopulations evolved over time from the parental strain by acquiring and accumulating resistance-conferring mutations to isoniazid, rifampin, and streptomycin. Overall, these observations indicate that during a chronic infection, several subpopulations may coexist in the same patient with different drug susceptibility profiles.
引用
收藏
页码:3114 / 3120
页数:7
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