Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection

被引：61

作者：

Ness, JM

Akhtar, RS

Latham, CB

Roth, KA

机构：

[1] Univ Alabama, Dept Pathol, Div Neuropathol, Sparks Ctr 961E, Birmingham, AL 35294 USA

[2] Univ Alabama, Dept Pediat, Div Pediat Neurol, Birmingham, AL 35294 USA

[3] Univ Alabama, Dept Neurobiol, Birmingham, AL 35294 USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2003年 / 51卷 / 08期

关键词：

immunohistochemistry; photobleaching; tyramide signal amplification; nanocrystals;

D O I：

10.1177/002215540305100801

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.

引用

收藏

页码：981 / 987

页数：7

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