An approach to the evaluation and comparison of reversed-phase high-performance liquid chromatography stationary phases

被引：9

作者：

Law, B ^{[1
]}

Houghton, SJ ^{[1
]}

Ballard, P ^{[1
]}

机构：

[1] Zeneca Pharmaceut, Drug Kinet Grp, Dept Safety Med, Macclesfield SK10 4TG, Cheshire, England

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1998年 / 17卷 / 03期

关键词：

HPLC; stationary phase evaluation; base-deactivated; data analysis; peak efficiency; peak asymmetry; selectivity; extra column band broadening;

D O I：

10.1016/S0731-7085(97)00214-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An approach to the evaluation and comparison of reversed-phase high-performance liquid chromatography stationary phases with particular emphasis on data analysis and presentation is described. Assessment is based on the peak efficiency, asymmetry (USP tailing factor) and relative retention properties shown by 24 basic compounds having a wide range of structural and physico-chemical properties. A novel approach-to data normalisation and presentation is described. This overcomes the problems associated with the quality of the column packing process, as well as differences in stationary phase selectivity which in conjunction with extra column band broadening effects can make comparisons meaningless. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

引用

页码：443 / 453

页数：11

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