Sensitivity-enhanced 2D IPAP, TROSY-anti-TROSY, and E.COSY experiments:: alternatives for measuring dipolar 15N-1HN couplings

被引：33

作者：

Ding, KY ^{[1
]}

Gronenborn, AM ^{[1
]}

机构：

[1] NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF MAGNETIC RESONANCE | 2003年 / 163卷 / 02期

基金：

美国国家卫生研究院;

关键词：

residual dipolar couplings; IPAP; TROSY-anti-TROSY; E.COSY; sensitivity enhancement;

D O I：

10.1016/S1090-7807(03)00081-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sensitivity-enhanced versions of the IPAP, TROSY-anti-TROSY, and E.COSY experiments for measuring one-bond N-15-H-1(N) couplings are presented. Together with the previously developed sensitivity-enhanced E.COSY-type HSQC experiment they comprise a suite of sensitivity-enhanced experiments that allows one to chose the optimal spectrum for accurate measurement of one-bond N-15-H-1(N) residual dipolar couplings in proteins. Since one-bond N-15-H-1(N) residual dipolar couplings play uniquely important roles in structural NMR, these additional methods provide further tools for improving structure determination of proteins and other biological macromolecules. Published by Elsevier Science (USA).

引用

页码：208 / 214

页数：7

共 22 条

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