Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid

被引:123
作者
Smith, JC
Lee, KB
Wang, Q
Finn, MG
Johnson, JE
Mrksich, M
Mirkin, CA
机构
[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[2] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
[3] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
[4] Northwestern Univ, Inst Nanotechnol, Evanston, IL 60208 USA
[5] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[6] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
关键词
SELF-ASSEMBLED MONOLAYERS; DIP-PEN NANOLITHOGRAPHY; SUPRAMOLECULAR BUILDING-BLOCKS; PLASMON RESONANCE SPECTROSCOPY; SELECTIVE IMMOBILIZATION; PROTEIN-PATTERNS; GOLD; FABRICATION; MICROARRAYS; ADSORPTION;
D O I
10.1021/nl025956h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary ink-consisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one malelmide group-to pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.
引用
收藏
页码:883 / 886
页数:4
相关论文
共 28 条
  • [1] Micropatterned surfaces for control of cell shape, position, and function
    Chen, CS
    Mrksich, M
    Huang, S
    Whitesides, GM
    Ingber, DE
    [J]. BIOTECHNOLOGY PROGRESS, 1998, 14 (03) : 356 - 363
  • [2] CHEN CS, 1997, SCIENCE, V276, P1345
  • [3] Nantotechniques and approaches in biotechnology
    Curtis, A
    Wilkinson, C
    [J]. TRENDS IN BIOTECHNOLOGY, 2001, 19 (03) : 97 - 101
  • [4] Direct patterning of modified oligonucleotides on metals and insulators by dip-pen nanolithography
    Demers, LM
    Ginger, DS
    Park, SJ
    Li, Z
    Chung, SW
    Mirkin, CA
    [J]. SCIENCE, 2002, 296 (5574) : 1836 - 1838
  • [5] Gradients of substrate-bound laminin orient axonal specification of neurons
    Dertinger, SKW
    Jiang, XY
    Li, ZY
    Murthy, VN
    Whitesides, GM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 12542 - 12547
  • [6] Dike LE, 1999, IN VITRO CELL DEV-AN, V35, P441
  • [7] Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands
    Hodneland, CD
    Lee, YS
    Min, DH
    Mrksich, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) : 5048 - 5052
  • [8] Multiple ink nanolithography: Toward a multiple-pen nano-plotter
    Hong, SH
    Zhu, J
    Mirkin, CA
    [J]. SCIENCE, 1999, 286 (5439) : 523 - 525
  • [9] Maleimide-functionalized self-assembled monolayers for the preparation of peptide and carbohydrate biochips
    Houseman, BT
    Gawalt, ES
    Mrksich, M
    [J]. LANGMUIR, 2003, 19 (05) : 1522 - 1531
  • [10] Carbohydrate arrays for the evaluation of protein binding and enzymatic modification
    Houseman, BT
    Mrksich, M
    [J]. CHEMISTRY & BIOLOGY, 2002, 9 (04): : 443 - 454