Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei

被引:344
作者
Foreman, PK
Brown, D
Dankmeyer, L
Dean, R
Diener, S
Dunn-Coleman, NS
Goedegebuur, F
Houfek, TD
England, GJ
Kelley, AS
Meerman, HJ
Mitchell, T
Mitchinson, C
Olivares, HA
Teunissen, PJM
Yao, J
Ward, M
机构
[1] Genencor Int Inc, Palo Alto, CA 94304 USA
[2] N Carolina State Univ, Fungal Genom Lab, Raleigh, NC 27695 USA
关键词
D O I
10.1074/jbc.M304750200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The filamentous fungus Trichoderma reesei produces and secretes profuse quantities of enzymes that act synergistically to degrade cellulase and related biomass components. We partially sequenced over 5100 random T. reesei cDNA clones. Among the sequences whose predicted gene products had significant similarity to known proteins, 12 were identified that encode previously unknown enzymes that likely function in biomass degradation. Microarrays were used to query the expression levels of each of the sequences under different conditions known to induce cellulolytic enzyme synthesis. Most of the genes encoding known and putative biomass-degrading enzymes were transcriptionally coregulated. Moreover, despite the fact that several of these enzymes are not thought to degrade cellulase directly, they were coordinately overexpressed in a cellulase overproducing strain. A variety of additional sequences whose function could not be ascribed using the limited sequence available displayed analogous behavior and may also play a role in biomass degradation or in the synthesis of biomass-degrading enzymes. Sequences exhibiting additional regulatory patterns were observed that might reflect roles in regulation of cellulase biosynthesis. However, genes whose products are involved in protein processing and secretion were not highly regulated during cellulase induction.
引用
收藏
页码:31988 / 31997
页数:10
相关论文
共 63 条
[51]  
SHEIRNEISS G, 1984, APPL MICROBIOL BIOT, V20, P46
[52]   MOLECULAR-CLONING OF EXO-CELLOBIOHYDROLASE-I DERIVED FROM TRICHODERMA-REESEI STRAIN-L27 [J].
SHOEMAKER, S ;
SCHWEICKART, V ;
LADNER, M ;
GELFAND, D ;
KWOK, S ;
MYAMBO, K ;
INNIS, M .
BIO-TECHNOLOGY, 1983, 1 (08) :691-696
[53]  
STALBRAND H, 1995, APPL ENVIRON MICROB, V61, P1090
[54]   Induction and catabolite repression mechanisms of cellulase in fungi [J].
Suto, M ;
Tomita, F .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2001, 92 (04) :305-311
[55]   Molecular cloning and expression of the novel fungal β-glucosidase genes from Humicola grisea and Trichoderma reesei [J].
Takashima, S ;
Nakamura, A ;
Hidaka, M ;
Masaki, H ;
Uozumi, T .
JOURNAL OF BIOCHEMISTRY, 1999, 125 (04) :728-736
[56]   HOMOLOGOUS DOMAINS IN TRICHODERMA-REESEI CELLULOLYTIC ENZYMES - GENE SEQUENCE AND EXPRESSION OF CELLOBIOHYDROLASE-II [J].
TEERI, TT ;
LEHTOVAARA, P ;
KAUPPINEN, S ;
SALOVUORI, I ;
KNOWLES, J .
GENE, 1987, 51 (01) :43-52
[57]   THE 2 MAJOR XYLANASES FROM TRICHODERMA-REESEI - CHARACTERIZATION OF BOTH ENZYMES AND GENES [J].
TORRONEN, A ;
MACH, RL ;
MESSNER, R ;
GONZALEZ, R ;
KALKKINEN, N ;
HARKKI, A ;
KUBICEK, CP .
BIO-TECHNOLOGY, 1992, 10 (11) :1461-1465
[58]  
UDENFRIEND S, 1995, METHOD ENZYMOL, V250, P571
[59]  
Ward M., 1993, BIOCH GENETICS CELLU, P53
[60]  
Wootton JC, 1996, METHOD ENZYMOL, V266, P554