Large-scale production of the carbohydrate portion of the sialyl-Tn epitope, α-Neup5Ac-(2→6)-D-GalpNAc, through bacterial coupling

被引：30

作者：

Endo, T ^{[1
]}

Koizumi, S ^{[1
]}

Tabata, K ^{[1
]}

Kakita, S ^{[1
]}

Ozaki, A ^{[1
]}

机构：

[1] Kyowa Hakko Kogyo Co Ltd, Tokyo Res Labs, Machida, Tokyo 1948533, Japan

来源：

CARBOHYDRATE RESEARCH | 2001年 / 330卷 / 04期

关键词：

enzymatic synthesis; glycosyltransferase; sugar nucleotide; oligosaccharide;

D O I：

10.1016/S0008-6215(01)00007-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha -Neup5Ac-(2 --> 6)-D-GalpNAc, the carbohydrate portion of sialyl-Tn epitope of the tumor-associated carbohydrate antigen, was prepared by a whole-cell reaction through the combination of recombinant Escherichia coli strains and Corynebacterium ammoniagenes. Two recombinant E. coli strains overexpressed the CMP-Neup 5Ac biosynthetic genes and the alpha-(2 --> 6)-sialyltransferase gene of Photobacterium damsela. C. ammoniagenes contributed to the production of UTP from orotic acid. alpha -NeupSAc-(2 --> 6)-D-GalpNAc was accumulated at 87 mM (45 g/L) after a 25-h reaction starting from orotic acid, N-acetylneuraminic acid, and 2-acetamide-2-deoxy-D-galactose. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：439 / 443

页数：5

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