Inhibitory effect of hot-water extract from dried fruit of Crataegus pinnatifida on low-density lipoprotein (LDL) oxidation in cell and cell-free systems

被引:55
作者
Chu, CY
Lee, MJ
Liao, CL
Lin, WL
Yin, YF
Tseng, TH
机构
[1] Chung Shan Med Univ, Sch Appl Chem, Coll Hlth Management, Taichung 402, Taiwan
[2] Chung Shan Med Univ, Coll Med, Inst Biochem, Taichung 402, Taiwan
[3] Chung Shan Med Univ Hosp, Dept Pathol, Taichung, Taiwan
关键词
Crataegus pinnatifida; antioxidation; low-density lipoprotein; RAW; 264.7; cells;
D O I
10.1021/jf030407y
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The dried fruit of Crataegus pinnatifida, a local soft drink material and medical herb, was found to possess potential against oxidative stress. In the preliminary study, the antioxidant potential of a hot-water extract obtained from the dried fruit of C. pinnatifida (CF-H) was evaluated in terms of its capacity of quenching 1,1 -diphenyl-2-picrylhydrazyl free radicals (EC50 = 0.118 mg/mL). After content analysis, it was found that CF-H is mainly composed of polyphenols including flavonoids (6.9%), procyanidins (2.2%), (+)-catechin (0.5%), and (-)-epicatechin (0.2%). The antioxidative bioactivity of CF-H had been assess previously using the models Of CuSO4 as cell-free system and sodium nitroprusside (SNP) plus macrophage RAW 264.7 cells as cell system to induce human low-density lipoprotein oxidation. CF-H was found to inhibit relative electrophoretic mobility and thiobarbituric acid reactive substances at the concentration of 0.5-1.0 mg/mL in the cell-free system and at 0.01-0.10 mg/mL in the cell system. Furthermore, it was found that CF-H decreased the SNP-induced cell lipid peroxidation and reduced glutathione depletion.
引用
收藏
页码:7583 / 7588
页数:6
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