The Pro-Apoptotic Protein Bim Is a MicroRNA Target in Kidney Progenitors

被引：80

作者：

Ho, Jacqueline ^{[2
,3
]}

Pandey, Priyanka ^{[2
,3
]}

Schatton, Tobias ^{[2
,4
,5
]}

Sims-Lucas, Sunder ^{[6
]}

Khalid, Myda ^{[2
,3
]}

Frank, Markus H. ^{[2
,4
,5
]}

Hartwig, Sunny ^{[2
,3
]}

Kreidberg, Jordan A. ^{[1
,2
,3
]}

机构：

[1] Harvard Univ, Childrens Hosp Boston, Sch Med, Dept Med,Div Nephrol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA

[3] Harvard Stem Cell Inst, Cambridge, MA USA

[4] Harvard Univ, Childrens Hosp Boston, Sch Med, Transplantat Res Ctr, Boston, MA 02115 USA

[5] Harvard Univ, Brigham & Womens Hosp, Sch Med, Boston, MA 02115 USA

[6] Univ Pittsburgh, Sch Med, Dept Pediat, Div Nephrol, Pittsburgh, PA 15261 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY | 2011年 / 22卷 / 06期

关键词：

PROCESSING ENZYME DICER; POLYCYSTIC KIDNEY; BCL-2; DEFICIENCY; EXPRESSION; DIFFERENTIATION; DELETION; MORPHOGENESIS; FAMILY; LEADS; MICE;

D O I：

10.1681/ASN.2010080841

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Understanding the mechanisms that regulate nephron progenitors during kidney development should aid development of therapies for renal failure. MicroRNAs, which modulate gene expression through post-transcriptional repression of specific target mRNAs, contribute to the differentiation of stem cells, but their role in nephrogenesis is incompletely understood. Here, we found that the loss of miRNAs in nephron progenitors results in a premature depletion of this population during kidney development. Increased apoptosis and expression of the pro-apoptotic protein Bim accompanied this depletion. Profiling of miRNA expression during nephrogenesis identified several highly expressed miRNAs (miR-10a, nniR-106b, miR-17-5p) in nephron progenitors that are either known or predicted to target Bim. We propose that modulation of apoptosis by miRNAs may determine congenital nephron endowment. Furthermore, our data implicate the pro-apoptotic protein Bim as a miRNA target in nephron progenitors.

引用

页码：1053 / 1063

页数：11

共 67 条

[11] miChip: A microarray platform for expression profiling of microRNAs based on locked nucleic acid (LNA) oligonucleotide capture probes [J].

Castoldi, Mirco ;

Benes, Vladimir ;

Hentze, Matthias W. ;

Muckenthaler, Martina U. .

METHODS, 2007, 43 (02) :146-152

[12] Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure [J].

Chen, Jian-Fu ;

Murchison, Elizabeth P. ;

Tang, Ruhang ;

Callis, Thomas E. ;

Tatsuguchi, Mariko ;

Deng, Zhongliang ;

Rojas, Mauricio ;

Hammond, Scott M. ;

Schneider, Michael D. ;

Selzman, Craig H. ;

Meissner, Gerhard ;

Patterson, Cam ;

Hannon, Gregory J. ;

Wang, Da-Zhi .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (06) :2111-2116

[13] BCL-2 family proteins: Critical checkpoints of apoptotic cell death [J].

Danial, Nika N. .

CLINICAL CANCER RESEARCH, 2007, 13 (24) :7254-7263

[14] The cellular basis of kidney development [J].

Dressler, Gregory R. .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2006, 22 :509-529

[15] Distinctive patterns of microRNA expression in primary muscular disorders [J].

Eisenberg, Iris ;

Eran, Alal ;

Nishino, Ichizo ;

Moggio, Maurizio ;

Lamperti, Costanza ;

Arnato, Anthony A. ;

Lidov, Hart G. ;

Kang, Peter B. ;

North, Kathryn N. ;

Mitrani-Rosenbaum, Stella ;

Flanigan, Kevin M. ;

Neely, Lori A. ;

Whitney, Duncan ;

Beggs, Alan H. ;

Kohane, Isaac S. ;

Kunkel, Louis M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (43) :17016-17021

[16] Bim and the pro-survival bcl-2 proteins - Opposites attract, ERK repels [J].

Ewings, Katherine E. ;

Wiggins, Ceri M. ;

Cook, Simon J. .

CELL CYCLE, 2007, 6 (18) :2236-2240

[17] miR-17 family miRNAs are expressed during early mammalian development and regulate stem cell differentiation [J].

Foshay, Kara M. ;

Gallicano, G. Ian .

DEVELOPMENTAL BIOLOGY, 2009, 326 (02) :431-443

[18] MicroRNAs in normal and malignant hematopoiesis [J].

Garzon, Ramiro ;

Croce, Carlo M. .

CURRENT OPINION IN HEMATOLOGY, 2008, 15 (04) :352-358

[19] MicroRNA targeting specificity in mammals: Determinants beyond seed pairing [J].

Grimson, Andrew ;

Farh, Kyle Kai-How ;

Johnston, Wendy K. ;

Garrett-Engele, Philip ;

Lim, Lee P. ;

Bartel, David P. .

MOLECULAR CELL, 2007, 27 (01) :91-105

[20] The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb [J].

Harfe, BD ;

McManus, MT ;

Mansfield, JH ;

Hornstein, E ;

Tabin, CJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (31) :10898-10903

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