On-line detection of antioxidative activity in high-performance liquid chromatography eluates by chemiluminescence

被引：66

作者：

Dapkevicius, A ^{[1
]}

van Beek, TA ^{[1
]}

Niederländer, HAG ^{[1
]}

de Groot, A ^{[1
]}

机构：

[1] Agr Univ Wageningen, Organ Chem Lab, NL-6703 HB Wageningen, Netherlands

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 03期

关键词：

D O I：

10.1021/ac9805908

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

Luminol chemiluminescence (CL) was employed for the on-line detection of radical scavengers in HPLC eluates. Optimization of CL reagents and instrumental setup resulted in a steady postcolumn luminol photochemical reaction in the presence of microperoxidase and hydrogen peroxide at pH 10. Quenching of the CL signal was utilized to detect radical scavenging activity of both natural and synthetic antioxidants at the nanogram level. The detection system can be used with isocratic or gradient elution. Several antioxidative compounds were detected in thyme and sage acetone extracts. Quantitative results can be obtained when antioxidants are analyzed at certain concentrations. The method is suitable for rapid screening of antioxidants in crude extracts.

引用

页码：736 / 740

页数：5

共 29 条

[1]

THE EFFECT OF PRIMARY ANTIOXIDANTS AND SYNERGISTS ON THE ACTIVITY OF PLANT-EXTRACTS IN LARD [J].