Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger

被引：26

作者：

Kirimura, K

Matsui, T

Sugano, S

Usami, S

机构：

[1] Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo

[2] Novo Nordisk Bioindustry Co. Ltd., Chiba 261-01, Nakase, Mihama-ku, Chiba-shi

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 141卷 / 2-3期

关键词：

alternative oxidase; antimycin A; Aspergillus niger; cyanide-insensitive respiration; salicylhydroxamic acid;

D O I：

10.1016/0378-1097(96)00230-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.

引用

页码：251 / 254

页数：4

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