Engineered enzymes for improved organic synthesis

被引:96
作者
Hult, K [1 ]
Berglund, P [1 ]
机构
[1] Royal Inst Technol, AlbaNova Univ Ctr, Dept Biotechnol, SE-10691 Stockholm, Sweden
关键词
D O I
10.1016/S0958-1669(03)00095-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Recent developments to modify enzymes for use in organic synthesis have targeted several areas. These include altering the reaction mechanism of the enzyme to catalyse new reactions, switching substrate specificity, expanding substrate specificity, and improving substrate specificity, such as enantioselectivity in kinetic resolutions. Such modifications can be achieved either by rational redesign, which requires knowledge of the enzyme structure, or by random mutagenesis methods followed by screening. Both strategies of enzyme engineering can be successful and are very useful for improving the utility of enzymes for applied catalysis. Several examples illustrating these concepts in a variety of enzyme classes have appeared recently.
引用
收藏
页码:395 / 400
页数:6
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