An α/β-HSQC-α/β experiment for spin-state selective editing of IS cross peaks

A generalized version of the TROSY experiment allows the spin-state selective editing of the four multiplet components of N-15-H-1 cross peaks of amide groups in proteins into four different subspectra, with no penalty in sensitivity. An improvement by root 2 in sensitivity results, if only two of the four multiplet components are selected. Use of the experiment for the measurement of 1J(HN) coupling constants is discussed. A water flip-back version of the experiment is demonstrated with a 45 kDa fragment of N-15/H-2 labeled Staphylococcus aureus gyrase B. (C) 1998 Academic Press.