Physiological 3D tissue model of the airway wall and mucosa

被引:58
作者
Choe, Melanie M.
Tomei, Alice A.
Swartz, Melody A. [1 ]
机构
[1] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA
[2] Ecole Polytech Fed Lausanne, Inst Bioengn, CH-1015 Lausanne, Switzerland
关键词
D O I
10.1038/nprot.2006.54
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This protocol describes the setup, maintenance and characteristics of a tissue-engineered model of the human bronchial mucosa that can be used for basic physiology and pathophysiology studies. The model includes a well-differentiated epithelium with functional cilia, mucus secretion and subepithelial fibroblasts within type I collagen. The tissue is created within porous polymeric wells to prevent gel contraction and allow culture at the air-liquid interface. It requires at least 2 wk to be established and can be maintained thereafter for over 4 wk, with tissue differentiation moving towards a more physiologically relevant phenotype with increasing time in culture. Over time, the extracellular matrix also remodels, depositing proteins such as types III and IV collagen and fibronectin. Because it recapitulates many key anatomical and functional features of the airway wall, this model is well suited for a wide range of studies, including those on airway remodeling, transepithelial transport and inflammatory cell interactions with the mucosa. The entire protocol takes 4 - 6 wk, including cell expansion, depending on the extent of ciliogenesis desired.
引用
收藏
页码:357 / 362
页数:6
相关论文
共 19 条
[1]  
Agarwal AR, 2003, ALLERGY ASTHMA PROC, V24, P35
[2]   PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO [J].
BELL, E ;
IVARSSON, B ;
MERRILL, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (03) :1274-1278
[3]   Bronchial mucose produced by tissue engineering:: A new tool to study cellular interactions in asthma [J].
Chakir, J ;
Pagé, N ;
Hamid, Q ;
Laviolette, M ;
Boulet, LP ;
Rouabhia, M .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2001, 107 (01) :36-40
[4]   An in vitro airway wall model of remodeling [J].
Choe, MM ;
Sporn, PHS ;
Swartz, MA .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2003, 285 (02) :L427-L433
[5]  
CHOE MM, 2006, AM J RESP CELL MOL B
[6]   Embryonic stem cells generate airway epithelial tissue [J].
Coraux, C ;
Nawrocki-Raby, A ;
Hinnrasky, J ;
Kileztky, C ;
Gaillard, D ;
Dani, C ;
Puchelle, E .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2005, 32 (02) :87-92
[7]   Cell interactions with three-dimensional matrices [J].
Cukierman, E ;
Pankov, R ;
Yamada, KM .
CURRENT OPINION IN CELL BIOLOGY, 2002, 14 (05) :633-639
[8]   CILIOGENESIS IN HUMAN BRONCHIAL EPITHELIAL-CELLS CULTURED AT THE AIR-LIQUID INTERFACE [J].
DEJONG, PM ;
VANSTERKENBURG, MAJA ;
HESSELING, SC ;
KEMPENAAR, JA ;
MULDER, AA ;
MOMMAAS, AM ;
DIJKMAN, JH ;
PONEC, M .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1994, 10 (03) :271-277
[9]   Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells [J].
Gray, TE ;
Guzman, K ;
Davis, CW ;
Abdullah, LH ;
Nettesheim, P .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1996, 14 (01) :104-112
[10]   Capturing complex 3D tissue physiology in vitro [J].
Griffith, LG ;
Swartz, MA .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2006, 7 (03) :211-224